周期素G抗體
產品名稱： 周期素G抗體
英文名稱： Cyclin G
產品編號： 1389
產品價格： null
產品產地： 上海
品牌商標： 雅吉
更新時間： null
使用范圍： WB ELISA IHC-P IHC-F IF
上海雅吉生物科技有限公司
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| 中文名稱 | 周期素G抗體 |
| 別????名 | CCNG 1; CCNG; CCNG1; CYCG1; Cyclin G; CCNG1_HUMAN; Cyclin-G; Cyclin G1; CyclinG1; Cyclin-G1.?? |
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| 研究領域 | 細胞生物??染色質和核信號??細胞周期蛋白??表觀遺傳學?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,? (predicted: Rat,?Chicken,?Dog,?Pig,?Cow,?Horse,?Rabbit,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 34kDa |
| 細胞定位 | 細胞核? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human Cyclin G:181-295/295? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive. Function: May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity). Subcellular Location: Nucleus. DNA replication foci after DNA damage. Tissue Specificity: High levels in skeletal muscle, ovary, kidney and colon. Similarity: Belongs to the cyclin family. Cyclin G subfamily. SWISS: P51959 Gene ID: 900 Database links: Entrez Gene: 900?Human Omim: 601578?Human SwissProt: P51959?Human Unigene: 79101?Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Cyclin G的生物功能還沒有完全清楚，他是細胞周期蛋白家族中近來發現的新成員，也是已知的唯一受到腫瘤抑制基因p53調控的細胞周期蛋白。 近年來經研究發現，Cyclin G可能有促進細胞增長的作用，在細胞增殖和神經再生中的可能起到一定的作用。 |
| 產品圖片 |  Sample:Liver (Mouse) Lysate at 40 ug Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34/30 kD  Sample:Kidney (Mouse) Lysate at 40 ug Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34/30 kD  Sample:Spleen (Mouse) Lysate at 40 ug Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34/30 kD  Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin G Polyclonal Antibody, Unconjugated(bs-1389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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