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Disease:

 Esophageal squamous cell carcinoma 

Origin:

 Established from poorly differentiated human esophageal squamous carcinoma 

Species:

Homo sapiens 

Tissue:

Esophagus 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Patient:

Medium:

RPMI1640 + 10% FBS 

Subculture:

1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Related cells by Organ:

EC-109 cells 

HKESC-1 cells 

OE33 cells 

JROECL33 cells 

NE-1 cells 


References for EC-109 cells:

1. Cai, X. B., Jing, X. B., Hu, H., Chen, S. Z., and Li, Y. H. [The role of reactive oxygen species in cisplatin-induced apoptosis of esophageal cancer cell line EC-109]. Ai Zheng, 25: 427-431, 2006. 

2. Jing, X. B., Cai, X. B., Hu, H., Chen, S. Z., Chen, B. M., and Cai, J. Y. Reactive oxygen species and mitochondrial membrane potential are modulated during CDDP-induced apoptosis in EC-109 cells. Biochem Cell Biol, 85: 265-271, 2007. 

3. Wang, Q. J., Cai, X. B., Liu, M. H., Hu, H., Tan, X. J., and Jing, X. B. Apoptosis induced by emodin is associated with alterations of intracellular acidification and reactive oxygen species in EC-109 cells. Biochem Cell Biol, 88: 767-774.