Anti-Rabbit IgG VHH Single Domain抗體(Sepharose)
產品名稱： Anti-Rabbit IgG VHH Single Domain抗體(Sepharose)
英文名稱： Anti-Rabbit IgG VHH Single Domain抗體(Sepharose)
產品編號： ab191867
產品價格： null
產品產地： 英國
品牌商標： abcam
更新時間： null
使用范圍：
- 聯系人 :
- 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
- 郵編 :
- 所在區域 : 廣東
- 電話 : 133****4454 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 1484332550@qq.com
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- 形式Liquid
- 存放說明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. Do Not Freeze.
- 存儲溶液pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, Sodium phosphate, Sodium chloride - ?
- 純度Purified via His tag
- 純化說明This product is a recombinant protein produced in E. coli.
- 克隆單克隆
- 常規說明
Sepharose? is a registered trademark of GE Healthcare.
- 研究領域
- Secondary antibodies ?
- anti-Rabbit ?
- IgG ?
- Enzyme ?
- HRP
應用
Our?Abpromise guarantee?covers the use of?ab191867?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) 圖像
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Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)Lane 1:?Diluted Rabbit Sera (1 in 100)
Lane 2:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) + Rabbit Sera
Lane 3:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) IP Flow-through
Lane 4:? Blocked beads + Rabbit Sera
Lane 5:?Diluted Rabbit Sera (1 in 100)
Lane 6:?Protein A beads + Rabbit Sera
Lane 7:?Protein A beads IP Flow-through
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Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) ab191867 beads were used to pull down Rabbit IgG from Rabbit Sera. Protein A beads were used as a positive control and blocked beads as a negative. The gel was stained with Optiblot Blue (ab119211).
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Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)Lane 1:?2μg HeLa Lysate
Lane 2:?2μg?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) IP supernatant
Lane 3:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) pulling down B-tubulin (50kDa)
Lane 4:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) no IP ab, Negative Control
Lane 5:?2μg HeLa Lysate
Lane 6:?2μg Protein A beads IP supernatant
Lane 7:?Protein A beads pulling down B-tubulin (50kDa)
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Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) beads were used to pull down β-tubulin from HeLa cell lysate with rabbit anti-β-tubulin (ab6046). Protein A agarose beads were used as a positive control. The gel was transferred to Nitrocellulose membrane and probed with mouse anti-β-tubulin (ab101019) and anti-mouse HRP (ab97040).
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Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)Lane 1:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) + 50μg Rabbit IgG Control (ab46540)
Lane 2:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) IP Supernatant
Lane 3:?Protein A beads + 50μg Rabbit IgG Control (ab46540)
Lane 4:?Protein A beads IP Supernatant?
Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) ab191867 beads were used to pull down Rabbit IgG Control, 50μg (ab46540). Protein A beads were used as a positive control. The gel was stained with Optiblot Blue (ab119211).
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Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)Lane 1:?2μg HeLa Lysate
Lane 2:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) beads IP supernatant
Lane 3:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) pulling down GAPDH from HeLa lysate (37 kDa)
Lane 4:?Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867), no IP ab (negative control)
Lane 5:?2μg HeLa Lysate
Lane 6:?Protein A beads IP supernatant
Lane 7:?Protein A beads pulling down GAPDH from HeLa lysate (37 kDa)?
Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) beads were used to pull down GAPDH from?HeLa cell lysate with?rabbit anti-GAPDH (ab128915). Protein A agarose beads were used as a positive control. The gel was transferred to Nitrocellulose membrane and probed with mouse anti-GAPDH (ab8245) and anti-mouse HRP (ab97040).