女人与狗锁死了视频大全|霍金死亡过程恐怖视频|无节社团简谱季免费观看|六间房直播大厅|中文字幕欧美另类精品|久久人人玩人妻潮喷内射人人|亚洲国产一区二区三区在线观看

Molecular Characterization

?

Molecular Characterization: Recombinant hMCP-1 has a calculated MW of 8,685. DTT-reduced protein migrates as a 10 kDa polypeptide. The nonreduced protein migrates at 12 kDa. The expected amino-terminal QPDAI of recombinant hMCP-1 was verified by amino acid sequencing.

?

Purity

?

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hMCP-1. All lots are greater than 95% pure.

?

Bioactivity

?

The activity of hMCP-1 was determined using a THP-1 cell migration assay. The ED₅₀?of each lot is between 1-11 ng/ml.

?

Bioactivity

hMCP-1-induced migration of THP-1 cells was assessed. THP-1 cells were incubated in a 96-well transwell plate with increasing concentrations of hMCP-1 in the bottom chamber. After 2 hr, the number of THP-1 cells that migrated to the bottom chamber of the transwell was quantified by measuring DNA content using a fluorescent dye.

Coomassie Gel

The purity of recombinant hMCP-1 was determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hMCP-1 and staining overnight with Coomassie Blue.

Applications

?

Optimal concentration for the desired application should be determined by the user.

?

Endotoxin

?

Less than 0.01 ng endotoxin/1 μg hMCP-1.

?

Formulation

?

With carrier:?Lyophilized from a 0.22 μm filtered solution of hMCP-1 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hMCP-1.?Carrier free:?Lyophilized from a 0.22 μm filtered solution of hMCP-1 in 20 mM Tris, pH 7.2.

?

Background

?

?

?

MCP-1 (CCL2) is the first member of the C-C family of chemokines to be identified (1). C-C chemokines are characterized by two adjacent cysteine residues within the polypeptide, which form an intra-molecular disulfide bond. MCP-1 is a potent chemotactic factor for monocytes/macrophages, T cells and a subset of NK cells (1-4). The MCP-1 receptor, CCR2, is expressed as two splice isoforms, CCR2A and CCR2B, of which CCR2B is the predominant form (1). MCP-1 is secreted by adipocytes and appears to be one of many links between obesity, inflammation, and diabetes (1). MCP-1/CCR2 signaling appears to play a key role in γδ effector T cells recruitment and anti-tumor responses in a murine B16 melanoma model (2). Conversely, CCL2 expression is upregulated in many types of cancer and has been implicated in promoting tumor cell survival, proliferation, and tumor associated inflammation (4).

11946????? Human TL1A/TNFSF15 (hTL1A)

11996????? Mouse His6Interleukin-25/IL-17E (mHis6IL-25)

12296???? Human Fibroblast Growth Factor 9 (hFGF9)

12337???? Human His6Interleukin-25/IL-17E (hHis6IL-25)

12568???? Human His6IGFBP2

12579???? Human His6IGFBP3 (hHis6IGFBP3)

12670???? MouseHis6Interleukin-21 (mHis6IL-21)

12786???? Human Interleukin-6 Receptor α (hIL-6Rα)

12838???? Mouse MCP-1

12900???? Human MCP-1

3583?????? Human CD40 Ligand (hCD40L)

3897?????? Human Brain-Derived Neurotrophic Factor (BDNF)

4697?????? Human BMP2

4698?????? Mouse His6 Tumor Necrosis Factor-α (mHis6TNF-α)

5154?????? Human Latent Transforming Growth Factor β1 (hLatent TGF-β1)

5164?????? Human Interleukin-28A (hIL-28A/IFN-λ2)

5178?????? Mouse Tumor Necrosis Factor-α (mTNF-α)

5183?????? Human Interleukin-29 (hIL-29)

5191?????? Mouse Granulocyte Macrophage Colony Stimulating Factor (mGM-CSF)

5201?????? Mouse Interleukin-2 (mIL-2)

5204?????? Mouse Interleukin-1β (mIL-1β)

5208?????? Mouse Interleukin-4 (mIL-4)

5211?????? Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 )

5216?????? Mouse Interleukin-6 (mIL-6)

5217?????? Mouse Interleukin-7 (mIL-7)

5218?????? Human Neuregulin-1 (hNRG-1)

5221?????? Human β-Nerve Growth Factor (hβ-NGF)

5222?????? Mouse Interferon-γ (mIFN-γ)

5223?????? Mouse Stem Cell Factor (mSCF)

5224?????? Mouse Interleukin-22 (mIL-22)

5227?????? Mouse Interleukin-17A (mIL-17A)

5228?????? Mouse Macrophage Colony Stimulating Factor (mM-CSF)

5231?????? Mouse Transforming Growth Factor β1 (mTGF-β1)

5233?????? Human BAFF/TNFSF13B (hBAFF)

5234?????? Human FGF acidic (hFGF acidic)

5235?????? Human Betacellulin (hBTC)

5236?????? Human Interleukin-1α (hIL-1α)

5237?????? Human Neurotrophin-3 (hNT-3)

5238?????? Human Insulin-like Growth Factor II (hIGF-II)

5242?????? Mouse Interleukin-13 (mIL-13)

5261?????? Mouse Interleukin-10 (mIL-10)

5273?????? Mouse Interleukin-1α (mIL-1α)

5312?????? Human RANKL/TRANCE/TNFSF11 (hRANKL)

5314?????? Mouse Vascular Endothelial Growth Factor-120 (mVEGF120 )

5331?????? Mouse Epidermal Growth Factor (mEGF)

5358?????? Human Interleukin-10 (hIL-10) (mammalian derived)

5367?????? Human Oncostatin M (hOSM)

5371?????? Mouse Oncostatin M (mOSM)

5413?????? Human His6BAFF/TNFSF13B (hHis6 BAFF)

5414?????? Mouse Basic Fibroblast Growth Factor (mFGF basic/FGF2)

5452?????? Human His6Fas Ligand/TNFSF6 (hHis6FasL)

5454?????? Human Interleukin-2 (hIL-2) (mammalian derived)

5493?????? Human Epigen

5494?????? Human Epiregulin

5495?????? Human Transforming Growth Factor α (hTGF-α)

5592?????? Human Neurotrophin-4 (hNT-4)

5712?????? Human Stromal Cell-derived Factor 1β/CXCL12 (hSDF1β)

5717?????? Human Cystatin C (hCystatin C)

5719?????? Human Leptin/OB (hLeptin)

5725?????? Mouse His6Interleukin-27 (mHis6IL-27)

5874?????? Rat Vascular Endothelial Growth Factor-164 (rVEGF164 )

5924?????? Human Interleukin-5 (hIL-5)

6980?????? Human Erythropoietin (hEPO)

6983?????? Mouse His6Interleukin-33 (mHis6IL-33)

8012?????? Human His6Thymic Stromal Lymphopoietin (hHis6TSLP)

8065?????? Human Vascular Endothelial Growth Factor-165 (hVEGF165 )

8170?????? Human Interleukin-7 (hIL-7)

8199?????? Mouse Lipocalin-2 (mLCN2)

8230?????? Human Lymphotoxin-α/TNF-β/TNFSF1 (hLT-α)

8406?????? Human Transforming Growth Factor β2 (hTGF-β2)

8425?????? Human Transforming Growth Factor β3 (hTGF-β3)

8445?????? Human C-C Motif Chemokine 3 (hCCL3/MIP-1-α)

8460?????? Human His64-1BB Ligand/TNFSF9 (hHis64-1BBL)

8796?????? Human Interleukin-28B (hIL-28B/IFN-λ3)

8846?????? Human Interleukin-17A/F Heterodimer (hIL-17A/F)

8857?????? Mouse His6Interleukin-6 (mHis6IL-6)

8900?????? Human Interleukin-1β (hIL-1β)

8901?????? Human Interferon-γ (hIFN-γ)

8902?????? Human Tumor Necrosis Factor-α (hTNF-α)

8903?????? Human Interleukin-10 (hIL-10)

8904?????? Human Interleukin-6 (hIL-6)

8905?????? Human Interleukin-13 (hIL-13)

8906?????? Human Interleukin-17F (hIL-17F)

8907?????? Human Interleukin-2 (hIL-2)

8908?????? Human Vascular Endothelial Growth Factor-121 (hVEGF121 )

8910?????? Human Basic Fibroblast Growth Factor (hFGF basic/FGF2)

8911?????? Human Leukemia Inhibitory Factor (hLIF)

8912?????? Human Platelet-Derived Growth Factor BB (hPDGF-BB)

8913?????? Human Platelet-Derived Growth Factor AA (hPDGF-AA)

8915?????? Human Transforming Growth Factor β1 (hTGF-β1)

8916?????? Human Epidermal Growth Factor (hEGF)

8917?????? Human Insulin-like Growth Factor I (hIGF-I)

8918?????? Human Interleukin-3 (hIL-3)

8919?????? Human Interleukin-4 (hIL-4)

8920?????? Human Interleukin-21 (hIL-21)

8921?????? Human Interleukin-8 (hIL-8)

8922?????? Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF)

8923?????? Mouse Interleukin-3 (mIL-3)

8924?????? Human Fms-related Tyrosine Kinase 3 Ligand (hFLT3L)

8925?????? Human Stem Cell Factor (hSCF)

8927?????? Human Interferon-α1 (hIFN-α1)

8928?????? Human Interleukin-17A (hIL-17A)

8929?????? Human Macrophage Colony Stimulating Factor (hM-CSF)

8930?????? Human Granulocyte Colony Stimulating Factor (hG-CSF)

8931?????? Human Interleukin-22 (hIL-22)

9584?????? Mouse Interleukin-17B (mIL-17B)

9897?????? Mouse Insulin-like Growth Factor I (mIGF-I)