• 種屬反應性
  與反應:?Rat, Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ribophorin I aa 550 to the C-terminus. The exact sequence is proprietary.
  Database link:?P04843

• 陽性對照
  • WB: HeLa, BxPC-3, C6, and PC-12 whole cell lysates. Rat kidney and spleen lysates.
• 常規說明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存儲溶液Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
• 濃度100 μl 濃度為 0.531 mg/ml?40 μl 濃度為 0.531 mg/ml?
  
• 純度Protein A purified
• 克隆單克隆
• 克隆編號EPR17044(B)
• 同種型IgG
• 研究領域
  • Epigenetics and Nuclear Signaling
  ?
  • DNA / RNA
  ?
  • Translation
  ?
  • Ribosome

應用

靶標

• 功能Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
• 組織特異性Expressed in all tissues tested.
• 序列相似性Belongs to the OST1 family.
• 細胞定位Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Information by UniProt
• 數據庫鏈接
  • Entrez Gene: 6184?Human
  • Entrez Gene: 25596?Rat
  • Omim: 180470?Human
  • SwissProt: P04843?Human
  • SwissProt: P07153?Rat
  • Unigene: 518244?Human
  • Unigene: 603636?Human
  • Unigene: 4224?Rat
• 別名
  • D6Wsu137e antibody
  • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
  • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody

  see all

Anti-Ribophorin I antibody [EPR17044(B)] 圖像

• 

  Western blot - Anti-Ribophorin I antibody [EPR17044(B)] (ab197888)
  All lanes :?Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/20000 dilution
  
  Lane 1 :?BxPC-3 (human Pancreas adenocarcinoma ) whole cell lysate
  Lane 2 :?HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
  
  Lysates/proteins at 20 μg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size :?69 kDa
  
  
  Exposure time :?1 minute

  Blocking and diluting buffer:?5% NFDM/TBST

• 

  Western blot - Anti-Ribophorin I antibody [EPR17044(B)] (ab197888)
  All lanes :?Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/2000 dilution
  
  Lane 1 :?Rat kidney lysate
  Lane 2 :?Rat spleen lysate
  Lane 3 :?C6 (Rat glial tumor cells) whole cell lysate
  Lane 4 :?PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
  
  Lysates/proteins at 10 μg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size :?69 kDa
  
  
  Exposure time :?3 minutes

  Blocking and diluting buffer:?5% NFDM/TBST