Hieff Trans?Polybrene(hexadimethrine bromide) 聚凝胺(10 mg/mL)
產(chǎn)品名稱： Hieff Trans?Polybrene(hexadimethrine bromide) 聚凝胺(10 mg/mL)
英文名稱： Hieff Trans?Polybrene(hexadimethrine bromide) 聚凝胺(10 mg/mL)
產(chǎn)品編號(hào)： 40804ES
產(chǎn)品價(jià)格： 詢價(jià)
產(chǎn)品產(chǎn)地： YEASEN
品牌商標(biāo)： YEASEN
更新時(shí)間： 2025-07-17T08:35:17
使用范圍： null
- 聯(lián)系人 : 李自轉(zhuǎn)
- 地址 : 上海市浦東新區(qū)天雄路166弄一號(hào)樓三層南單元
- 郵編 : 200030
- 所在區(qū)域 : 上海
- 電話 : 139****5640 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : lizizhuan@yeasen.com
- 二維碼 : 點(diǎn)擊查看
Polybrene（聚凝胺，hexadimethrine bromide）是一種多聚陽(yáng)離子聚合物，常用于哺乳動(dòng)物細(xì)胞的DNA轉(zhuǎn)染實(shí)驗(yàn)以增強(qiáng)脂質(zhì)體的轉(zhuǎn)染效率。Polybrene目前廣泛用于逆轉(zhuǎn)錄病毒介導(dǎo)的基因轉(zhuǎn)染，慢病毒介導(dǎo)的基因轉(zhuǎn)染，作用機(jī)理可能是通過(guò)中和細(xì)胞表面唾液酸與病毒顆粒之間的靜電排斥從而促進(jìn)吸附作用。Polybrene也是一種有名的抗肝素劑（肝素拮抗劑），常用來(lái)生產(chǎn)非特異性凝集的紅細(xì)胞。另外Polybrene也多用于蛋白測(cè)序，因?yàn)樾┝康腜olybrene在自動(dòng)測(cè)序分析可明顯改善多肽的降解現(xiàn)象。PVDF膜加入polybrene還能提高膜的親和性。
本產(chǎn)品以溶液形式提供，粉末用0.9% NaCl配制成10 mg/mL的溶液，并用0.22 μM濾膜過(guò)濾除菌。使用時(shí)一般按1:1000-1:2000稀釋，依細(xì)胞種類不同稀釋比例不同，具體查閱相關(guān)文獻(xiàn)。
提高轉(zhuǎn)染效率：Polybrene（聚凝胺）是一種陽(yáng)離子聚合物，能夠與病毒載體（如慢病毒）表面的負(fù)電荷相互作用，增加病毒顆粒對(duì)細(xì)胞的黏附，從而顯著提高病毒對(duì)細(xì)胞的感染效率。
穩(wěn)定性好：該產(chǎn)品在2-8℃的條件下保存，性能穩(wěn)定，有效期較長(zhǎng)，能夠保證在實(shí)驗(yàn)過(guò)程中提供可靠的效果。
操作簡(jiǎn)便：使用時(shí)無(wú)需復(fù)雜的預(yù)處理，可以直接按照一定的比例加入到含有病毒載體的培養(yǎng)基中，與其他轉(zhuǎn)染試劑或方法相比，操作步驟相對(duì)簡(jiǎn)單。
冰袋（wet ice）運(yùn)輸。-20 ºC可保存2年，建議分裝保存，避免反復(fù)凍融。
Q: 聚凝胺濃度是10mg/ml，用什么稀釋？
A: 聚凝胺稀釋不是指用其他溶液稀釋，而是指在具體實(shí)驗(yàn)中加入的比例，比如實(shí)驗(yàn)體系有10mL,按照這個(gè)比例（1：1000為例），就直接取10uL的產(chǎn)品（10mg/mL溶液）加入實(shí)驗(yàn)體系里面即可。
[1] Lu T, Zhang Z, Zhang J, et al. CD73 in small extracellular vesicles derived from HNSCC defines tumour-associated immunosuppression mediated by macrophages in the microenvironment. J Extracell Vesicles. 2022;11(5):e12218. doi:10.1002/jev2.12218(IF:25.841)
[2] Hu S, Peng L, Xu C, Wang Z, Song A, Chen FX. SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. Mol Cell. 2021;81(21):4425-4439.e6. doi:10.1016/j.molcel.2021.08.029(IF:17.970)
[3] Xiao R, You L, Zhang L, et al. Inhibiting the IRE1α Axis of the Unfolded Protein Response Enhances the Antitumor Effect of AZD1775 in TP53 Mutant Ovarian Cancer. Adv Sci (Weinh). 2022;9(21):e2105469. doi:10.1002/advs.202105469(IF:16.806)
[4] Wu Z, Liu J, Chen G, et al. CD146 is a Novel ANGPTL2 Receptor that Promotes Obesity by Manipulating Lipid Metabolism and Energy Expenditure. Adv Sci (Weinh). 2021;8(6):2004032. Published 2021 Jan 27. doi:10.1002/advs.202004032(IF:16.806)
[5] Tu H, Wang Z, Yuan Y, et al. The PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium. Nat Commun. 2022;13(1):1787. Published 2022 Apr 4. doi:10.1038/s41467-022-29503-1(IF:14.919)
[6] Han Z, Zhang W, Ning W, et al. Model-based analysis uncovers mutations altering autophagy selectivity in human cancer. Nat Commun. 2021;12(1):3258. Published 2021 May 31. doi:10.1038/s41467-021-23539-5(IF:14.919)
[7] Lv Y, Wang H, Li G, Zhao B. Three-dimensional decellularized tumor extracellular matrices with different stiffness as bioengineered tumor scaffolds. Bioact Mater. 2021;6(9):2767-2782. Published 2021 Feb 15. doi:10.1016/j.bioactmat.2021.02.004(IF:14.593)
[8] Liu P, Wang X, Sun Y, et al. SARS-CoV-2 ORF8 reshapes the ER through forming mixed disulfides with ER oxidoreductases. Redox Biol. 2022;54:102388. doi:10.1016/j.redox.2022.102388(IF:11.799)
[9] Zhang M, Liu F, Zhou P, et al. The MTOR signaling pathway regulates macrophage differentiation from mouse myeloid progenitors by inhibiting autophagy. Autophagy. 2019;15(7):1150-1162. doi:10.1080/15548627.2019.1578040(IF:11.059)
[10] Zhang Y, Huang YX, Jin X, et al. Overexpression of lncRNAs with endogenous lengths and functions using a lncRNA delivery system based on transposon. J Nanobiotechnology. 2021;19(1):303. Published 2021 Oct 2. doi:10.1186/s12951-021-01044-7(IF:10.435)
[11] Sun Q, Ye Y, Gui A, et al. MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma. Cancer Lett. 2022;537:215678. doi:10.1016/j.canlet.2022.215678(IF:8.679)
[12] Huang Q, Hsueh CY, Shen YJ, et al. Small extracellular vesicle-packaged TGFβ1 promotes the reprogramming of normal fibroblasts into cancer-associated fibroblasts by regulating fibronectin in head and neck squamous cell carcinoma. Cancer Lett. 2021;517:1-13. doi:10.1016/j.canlet.2021.05.017(IF:8.679)
[13] Xu J, Meng Q, Sun H, et al. HER2-specific chimeric antigen receptor-T cells for targeted therapy of metastatic colorectal cancer. Cell Death Dis. 2021;12(12):1109. Published 2021 Nov 27. doi:10.1038/s41419-021-04100-0(IF:8.469)
[14] Bei Y, Huang Z, Feng X, et al. Lymphangiogenesis contributes to exercise-induced physiological cardiac growth. J Sport Health Sci. 2022;11(4):466-478. doi:10.1016/j.jshs.2022.02.005(IF:7.179)
[15] Jia J, Li C, Luo S, et al. Yes-Associated Protein Contributes to the Development of Human Cutaneous Squamous Cell Carcinoma via Activation of RAS. J Invest Dermatol. 2016;136(6):1267-1277. doi:10.1016/j.jid.2016.02.005(IF:6.915)
[16] Wang J, Liu R, Mo H, Xiao X, Xu Q, Zhao W. Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B. Int J Biol Sci. 2021;17(13):3331-3342. Published 2021 Jul 25. doi:10.7150/ijbs.61128(IF:6.582)
[17] Wang Q, Bu S, Xin D, Li B, Wang L, Lai D. Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties. Oxid Med Cell Longev. 2018;2018:7010472. Published 2018 Sep 17. doi:10.1155/2018/7010472(IF:6.543)
[18] Lai MC, Zhu QQ, Xu J, Zhang WJ. Experimental and clinical evidence suggests that GRPEL2 plays an oncogenic role in HCC development. Am J Cancer Res. 2021;11(9):4175-4198. Published 2021 Sep 15. (IF:6.166)
[19] Xu C, Zhang M, Bian L, Li Y, Yao Y, Li D. N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells. Oncogenesis. 2020;9(1):4. Published 2020 Jan 8. doi:10.1038/s41389-019-0188-1(IF:6.119)
[20] Huang J, Zhou X, Wang W, et al. Combined analyses of RNA-sequence and Hi-C along with GWAS loci-A novel approach to dissect keloid disorder genetic mechanism. PLoS Genet. 2022;18(6):e1010168. Published 2022 Jun 16. doi:10.1371/journal.pgen.1010168(IF:5.917)
[21] Miao Y, Chen X, Qin M, Zhou W, Wang Y, Ji Y. lncRNA GAS5, as a ceRNA, inhibits the proliferation of diffuse large B?cell lymphoma cells by regulating the miR?18a?5p/RUNX1 axis. Int J Oncol. 2021;59(5):94. doi:10.3892/ijo.2021.5274(IF:5.650)
[22] Meng L, Yuan W, Chi H, et al. Genetic deletion of CMG2 exacerbates systemic-to-pulmonary shunt-induced pulmonary arterial hypertension [published correction appears in FASEB J. 2021 Jul;35(7):e21619]. FASEB J. 2021;35(4):e21421. doi:10.1096/fj.202000299R(IF:5.192)
[23] Xiao L, Peng Z, Zhu A, et al. Inhibition of RUNX1 promotes cisplatin-induced apoptosis in ovarian cancer cells. Biochem Pharmacol. 2020;180:114116. doi:10.1016/j.bcp.2020.114116(IF:4.960)
[24] Wu D, He J, Zhang W, et al. CARM1 promotes non-small cell lung cancer progression through upregulating CCNE2 expression. Aging (Albany NY). 2020;12(11):10578-10593. doi:10.18632/aging.103280(IF:4.831)
[25] Hou X, Yang L, Jiang X, et al. Role of microRNA-141-3p in the progression and metastasis of hepatocellular carcinoma cell. Int J Biol Macromol. 2019;128:331-339. doi:10.1016/j.ijbiomac.2019.01.144(IF:4.784)
[26] Xu X, Yang C, Yu X, Wang J. Fibulin-3 regulates the inhibitory effect of TNF-α on chondrocyte differentiation partially via the TGF-β/Smad3 signaling pathway. Biochim Biophys Acta Mol Cell Res. 2022;1869(9):119285. doi:10.1016/j.bbamcr.2022.119285(IF:4.739)
[27] Xiao X, Lai W, Xie H, et al. Targeting JNK pathway promotes human hematopoietic stem cell expansion. Cell Discov. 2019;5:2. Published 2019 Jan 8. doi:10.1038/s41421-018-0072-8(IF:4.600)
[28] Yang R, Yang E, Shen L, Modlin RL, Shen H, Chen ZW. IL-12+IL-18 Cosignaling in Human Macrophages and Lung Epithelial Cells Activates Cathelicidin and Autophagy, Inhibiting Intracellular Mycobacterial Growth [published correction appears in J Immunol. 2019 Oct 1;203(7):2020]. J Immunol. 2018;200(7):2405-2417. doi:10.4049/jimmunol.1701073(IF:4.539)
[29] Liu J, Chen X, Liu Y, et al. Characterization of SARS-CoV-2 worldwide transmission based on evolutionary dynamics and specific viral mutations in the spike protein. Infect Dis Poverty. 2021;10(1):112. Published 2021 Aug 21. doi:10.1186/s40249-021-00895-4(IF:4.388)
[30] Hong Z, Pan J, Chen M, et al. Long Intergenic Noncoding RNA 00641 Promotes Growth and Invasion of Colorectal Cancer through Regulating miR-450b-5p/GOLPH3 Axis. J Oncol. 2022;2022:8259135. Published 2022 Jun 15. doi:10.1155/2022/8259135(IF:4.375)
[31] Zhu L, Zhao W, Lu J, et al. Influenza virus matrix protein M1 interacts with SLD5 to block host cell cycle. Cell Microbiol. 2019;21(8):e13038. doi:10.1111/cmi.13038(IF:4.288)
[32] Zhu L, Li X, Xu H, et al. Multiple RNA virus matrix proteins interact with SLD5 to manipulate host cell cycle. J Gen Virol. 2021;102(12):001697. doi:10.1099/jgv.0.001697(IF:3.891)
[33] Fang X, Liu Y, Xiao W, et al. Prognostic SLC family genes promote cell proliferation, migration, and invasion in hepatocellular carcinoma. Acta Biochim Biophys Sin (Shanghai). 2021;53(8):1065-1075. doi:10.1093/abbs/gmab076(IF:3.848)
[34] Jin Y, Qin X. Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma. Int J Biochem Cell Biol. 2020;127:105846. doi:10.1016/j.biocel.2020.105846(IF:3.673)
[35] Weimin S, Abula A, Qianghong D, Wenguang W. Chimeric cytokine receptor enhancing PSMA-CAR-T cell-mediated prostate cancer regression. Cancer Biol Ther. 2020;21(6):570-580. doi:10.1080/15384047.2020.1739952(IF:3.659)
[36] Ge L, Tan W, Li G, Gong N, Zhou L. Circ_0026134 promotes NSCLC progression by the miR-3619-5p/CHAF1B axis. Thorac Cancer. 2022;13(4):582-592. doi:10.1111/1759-7714.14301(IF:3.500)
[37] Jin Y, Li Y, Wang X, Yang Y. Secretory leukocyte protease inhibitor suppresses HPV E6-expressing HNSCC progression by mediating NF-κB and Akt pathways. Cancer Cell Int. 2019;19:220. Published 2019 Aug 23. doi:10.1186/s12935-019-0942-7(IF:3.439)
[38] Song L, Duan X, Zeng X, Duan X, Li L. Regulatory Mechanism of LINC00152 on Aggravating Heart Failure through Triggering Fibrosis in an Infarcted Myocardium. Dis Markers. 2021;2021:2607358. Published 2021 Dec 1. doi:10.1155/2021/2607358(IF:3.434)
[39] Yang M, Gu J, Xu F, Wang Y, Wang H, Zhang B. The protective role of glucocerebrosidase/ceramide in rheumatoid arthritis [published online ahead of print, 2022 Mar 21]. Connect Tissue Res. 2022;1-9. doi:10.1080/03008207.2022.2055552(IF:3.417)
[40] Zhu X, Li M, Jia X, et al. The homeoprotein Msx1 cooperates with Pkn1 to prevent terminal differentiation in myogenic precursor cells. Biochimie. 2019;162:55-65. doi:10.1016/j.biochi.2019.04.003(IF:3.362)
[41] Zhou J, Ding J, Ma X, et al. The NRF2/KEAP1 Pathway Modulates Nasopharyngeal Carcinoma Cell Radiosensitivity via ROS Elimination. Onco Targets Ther. 2020;13:9113-9122. Published 2020 Sep 11. doi:10.2147/OTT.S260169(IF:3.337)
[42] Chen Y, Li X, Xu J, et al. Knockdown of nuclear receptor binding SET domain-containing protein 1 (NSD1) inhibits proliferation and facilitates apoptosis in paclitaxel-resistant breast cancer cells via inactivating the Wnt/β-catenin signaling pathway. Bioengineered. 2022;13(2):3526-3536. doi:10.1080/21655979.2021.2018973(IF:3.269)
[43] He J, Jin S, Zhang W, et al. Long non-coding RNA LOC554202 promotes acquired gefitinib resistance in non-small cell lung cancer through upregulating miR-31 expression. J Cancer. 2019;10(24):6003-6013. Published 2019 Oct 15. doi:10.7150/jca.35097(IF:3.182)
[44] Gu J, Wang L, Li T, et al. Role and mechanism of organic cation transporter 3 in oxaliplatin treatment of colon cancer in vitro and in vivo. Oncol Rep. 2019;42(4):1355-1364. doi:10.3892/or.2019.7267(IF:3.041)
[45] Yang M, Zhao Q, Zhao H, et al. Adipose?derived stem cells overexpressing SK4 calcium?activated potassium channel generate biological pacemakers. Int J Mol Med. 2019;44(6):2103-2112. doi:10.3892/ijmm.2019.4374(IF:2.928)
[46] Zhou JT, Liu JH, Song TT, Ma B, Amidula N, Bai C. EGLIF-CAR-T Cells Secreting PD-1 Blocking Antibodies Significantly Mediate the Elimination of Gastric Cancer. Cancer Manag Res. 2020;12:8893-8902. Published 2020 Sep 23. doi:10.2147/CMAR.S260915(IF:2.886)
[47] Zhang H, Zhao H, He X, Xi F, Liu J. JAK-STAT Domain Enhanced MUC1-CAR-T Cells Induced Esophageal Cancer Elimination. Cancer Manag Res. 2020;12:9813-9824. Published 2020 Oct 8. doi:10.2147/CMAR.S264358(IF:2.886)
[48] Tai Y, Li Y, Zhang M. Silencing of circ_OSBPL10 affects the functional behaviors of oral squamous cell carcinoma cells by the miR-299-3p/CDK6 axis. Arch Oral Biol. 2022;136:105363. doi:10.1016/j.archoralbio.2022.105363(IF:2.635)
[49] Zhao Z, Li Y, Liu W, Li X. Engineered IL-7 Receptor Enhances the Therapeutic Effect of AXL-CAR-T Cells on Triple-Negative Breast Cancer. Biomed Res Int. 2020;2020:4795171. Published 2020 Jan 2. doi:10.1155/2020/4795171(IF:2.276)
[50] Ren CF, Zhao YX, Hou CF, et al. Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression. Mol Med Rep. 2017;15(1):460-466. doi:10.3892/mmr.2016.5968(IF:1.692)
[51] Zhang J, Zhang Z. Mechanisms of circular RNA circ_0066147 on pancreatic cancer progression. Open Life Sci. 2021;16(1):495-510. Published 2021 May 22. doi:10.1515/biol-2021-0047(IF:0.938)