Hieff Trans?Polybrene(hexadimethrine bromide) 聚凝胺(10 mg/mL)
產品名稱: Hieff Trans?Polybrene(hexadimethrine bromide) 聚凝胺(10 mg/mL)
英文名稱: Hieff Trans?Polybrene(hexadimethrine bromide) 聚凝胺(10 mg/mL)
產品編號: 40804ES
產品價格: 詢價
產品產地: YEASEN
品牌商標: YEASEN
更新時間: 2025-07-17T08:35:17
使用范圍: null
- 聯系人 : 李自轉
- 地址 : 上海市浦東新區天雄路166弄一號樓三層南單元
- 郵編 : 200030
- 所在區域 : 上海
- 電話 : 139****5640 點擊查看
- 傳真 : 點擊查看
- 郵箱 : lizizhuan@yeasen.com
- 二維碼 : 點擊查看
Polybrene(聚凝胺,hexadimethrine bromide)是一種多聚陽離子聚合物,常用于哺乳動物細胞的DNA轉染實驗以增強脂質體的轉染效率。Polybrene目前廣泛用于逆轉錄病毒介導的基因轉染,慢病毒介導的基因轉染,作用機理可能是通過中和細胞表面唾液酸與病毒顆粒之間的靜電排斥從而促進吸附作用。Polybrene也是一種有名的抗肝素劑(肝素拮抗劑),常用來生產非特異性凝集的紅細胞。另外Polybrene也多用于蛋白測序,因為小劑量的Polybrene在自動測序分析可明顯改善多肽的降解現象。PVDF膜加入polybrene還能提高膜的親和性。
本產品以溶液形式提供,粉末用0.9% NaCl配制成10 mg/mL的溶液,并用0.22 μM濾膜過濾除菌。使用時一般按1:1000-1:2000稀釋,依細胞種類不同稀釋比例不同,具體查閱相關文獻。
提高轉染效率:Polybrene(聚凝胺)是一種陽離子聚合物,能夠與病毒載體(如慢病毒)表面的負電荷相互作用,增加病毒顆粒對細胞的黏附,從而顯著提高病毒對細胞的感染效率。
穩定性好:該產品在2-8℃的條件下保存,性能穩定,有效期較長,能夠保證在實驗過程中提供可靠的效果。
操作簡便:使用時無需復雜的預處理,可以直接按照一定的比例加入到含有病毒載體的培養基中,與其他轉染試劑或方法相比,操作步驟相對簡單。
冰袋(wet ice)運輸。-20 ºC可保存2年,建議分裝保存,避免反復凍融。
Q: 聚凝胺濃度是10mg/ml,用什么稀釋?
A: 聚凝胺稀釋不是指用其他溶液稀釋,而是指在具體實驗中加入的比例,比如實驗體系有10mL,按照這個比例(1:1000為例),就直接取10uL的產品(10mg/mL溶液)加入實驗體系里面即可。
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