Size??100ul
Cat code??200240-6C4
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Clone Number??6C4-C12-H10
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??53kDa
Sensitivity??This antibody detects endogenous levels of p53 and does not cross-react with related proteins.
Immunogen??Purified recombinant human p53 protein fragments expressed in E.coli.
UniProt Number??P04637
GeneBank ID??NM_000546
Subcellular location??Cytoplasm. Nucleus. Endoplasmic reticulum
Formulation??Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername??P53; LFS1; TRP53;TP53
Gene_symbol??P53; LFS1; TRP53; FLJ92943
Summary??This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it′s believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq, Jul 2008]
UniPort summary  Function??Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Application Images :??
Western blot detection of TP53 in SW480,T47D and MDA-MB-468 cell lysates using P53 antibody (1:1000 diluted).Predicted band size: 53KDa.Observed band size: 53KDa.