來源
用途
交叉反應性
理論分子量
實際分子量
Rabbit
WB, IP, IF
H, M, R
28/83kDa
82kDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
WB
IP
IF
IHC
ICC
FC
ChIP
ChIP-seq
1:500-1:2000
1:50-1:100
1:50-1:200
-
-
-
-
-
抗體詳細信息如下::
About this Antibody
Name
HADHA Rabbit Polyclonal Antibody (KO Validated)
Category
Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype
IgG
Purification method
Affinity purification
Positive samples
K-562, THP-1, SW480, PC-3, HeLa, Mouse intestine, Mouse heart, Mouse adipose
Cellular location
Mitochondrion
Customer validation
-
About the Immunogen
Immunogen
Recombinant fusion protein of human HADHA (NP_000173.2).
Sequence
IAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ
Gene ID
3030
Swiss Prot
P40939
Synonyms
HADHA; ECHA; GBP; HADH; LCEH; LCHAD; MTPA; TP-ALPHA; trifunctional enzyme subunit alpha, mitochondrial
Category
Fatty acid pathway
Background
HADHA (Trifunctional enzyme subunit alpha, mitochondrial), also known as TP-alpha, is the 763 amino acid alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. This mitochondrial complex is complosed of four alpha (HADHA) and four beta (HADHB) subunits, and the alpha subunit (HADHA) is responsible for catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in the HADHA gene can lead to long-chain 3-hydroxyacyl-coenzyme A dehydrogenase (LCHAD) deficiency or mitochondrial trifunctional protein deficiency. LCHAD deficiency is characterized by a deficiency of the dehydrogenase activity with normal hydratase activity and moderately decreased thiolase activity. In mitochondrial trifunctional protein deficiency, all three activities of the protein, dehydrogenase, hydratase, and thiolase, are deficient.
包裝清單：
產品編號
產品名稱
包裝
AF5177
HADHA Rabbit Polyclonal Antibody (KO Validated)
50μl
AZ050
Western一抗稀釋液
50ml
—
說明書
1份
保存條件：
HADHA Rabbit Polyclonal Antibody (KO Validated) -20oC保存，Western一抗稀釋液-20oC或4oC保存，一年有效。Western一抗稀釋液優先推薦4oC保存，長期不使用可以考慮-20oC保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品僅限于專業人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3. 其它實驗操作請自行參考適當的protocol進行。
4. 代表性圖片：
Fig. 1. Western blot analysis of extracts of various cell lines, using HADHA antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: QuickBlock? Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.
Fig. 2. Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: QuickBlock? Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.
Fig. 3. Immunofluorescence analysis of U2OS cells using HADHA antibody. Blue: DAPI for nuclear staining.
Fig. 4. Immunoprecipitation analysis of 200μg extracts of HeLa cells using 3ug HADHA antibody at a dilition of 1:1000.
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