DIG標記反應試劑
 
抗地高辛(DIG)堿性磷酸酶復合物Anti-Digoxigenin-AP, Fab fragments：from sheep
 
產品號
 產品規格
 廠商
 價格
 
1093274

11093274910
 150u(200ul)
 Roche
 2156
 

Anti-Digoxigenin-AP, Fab fragments

Application

For the detection of digoxigenin-labeled compounds. 

Dot Blot ELISA Immunohisto/cytochemistry In situ hybridization Northern blot Southern blot Western blot Product Description

Specificity: Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).
Working concentration:
Dot blot: 150 mU/ml
ELISA: 150 - 300 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml

Background Information

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Contents

Solution, stabilized