Anti-EGFR (vIII) Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-EGFR (vIII) Mouse Monoclonal Antibody

Anti-EGFR (vIII) Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-EGFR (vIII) Mouse Monoclonal Antibody

英文名稱： Anti-EGFR (vIII) Mouse Monoclonal Antibody

產品編號： 26176

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IF、IHC

武漢紐斯特生物技術有限公司
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EGFR (vIII) [26176]
[100 μL]		 ?

Catalog Number:??26176
Gene Symbol:??EGFR/HER1/ERBB1
Description: ??Anti-EGFR (vIII) Mouse Monoclonal Antibody
Background: ??EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC).
Immunogen: ??A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin.
Applications: ??ELISA, WB, IF, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:1000-1:5000?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes EGFR (vIII), but not wild type EGFR from vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant EGFR (vIII) and wildtype proteins. Purified His-tagged EGFR (vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR (vIII) mouse monoclonal antibody (Cat. # 26176).
Immunofluorescence:??
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (vIII) plasmid (right column), then fixed and stained with anti-EGFR (vIII) monoclonal antibody (Cat. #26176).
Publications:
1.??Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
????Tumor Biology. October 2014

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