• 靶標種屬Mouse
• 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
• 經測試應用ICC,?IHC-P,?ELISA,?WBmore details
• 偶聯物HRP

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C.
• 存儲溶液Preservative: None
  Constituents: 0.2% BSA, PBS
• 濃度1 mg 濃度為 1 mg/ml?
  
• 純度Immunogen affinity purified
• 純化說明Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
• 共軛說明Molar enzyme/ antibody protein ratio is 4:1
• 克隆多克隆
• 同種型IgG
• 研究領域
  • Immunology
  ?
  • Immunoglobulins
  ?
  • Heavy Chain
  ?
  • IgG

  • Secondary antibodies
  ?
  • anti-Mouse
  ?
  • IgG
  ?
  • Enzyme
  ?
  • HRP

應用

Goat Anti-Mouse IgG Fc (HRP) 圖像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (ab97265)

  IHC image of beta actin staining in normal human colon, formalin-fixed and?paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with?ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97265,? 1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is secondary-only at 1/200 dilution.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre