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中文名稱
磷酸核糖焦磷酸合成酶相關蛋白2抗體
別????名
41 kDa phosphoribosypyrophosphate synthetase associated protein; 41 kDa phosphoribosypyrophosphate synthetase-associated protein; KPRB; KPRB_HUMAN; MGC117304; MGC126719; MGC126721; PAP41; Phosphoribosyl pyrophosphate synthase-associated protein 2; Phosphoribosyl pyrophosphate synthetase associated protein 2; PRPP synthase-associated protein 2; PRPP synthetase associated protein 2; Prpsap2.??
研究領域
細胞生物??信號轉導??表觀遺傳學??
抗體來源
Rabbit
克隆類型
Polyclonal
交叉反應
Human,?Mouse,?Rat,? (predicted: Chicken,?Dog,?Horse,?Rabbit,?)
產品應用
WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?ICC=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分?子?量
41kDa
細胞定位
細胞核?
性????狀
Liquid
濃????度
1mg/ml
免?疫?原
KLH conjugated synthetic peptide derived from human PRPSAP2:1-100/369?
亞????型
IgG
純化方法
affinity purified by Protein A
儲?存?液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed
PubMed
產品介紹
This gene encodes a protein that associates with the enzyme phosphoribosylpyrophosphate synthetase (PRS). PRS catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Function:
Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the ribose-phosphate pyrophosphokinase family.

SWISS:
O60256

Gene ID:
5636

Database links:
Entrez Gene: 5636?Human
Entrez Gene: 212627?Mouse
Entrez Gene: 117272?Rat
Omim: 603762?Human
SwissProt: O60256?Human
SwissProt: Q8R574?Mouse
SwissProt: O08618?Rat
Unigene: 632236?Human
Unigene: 27703?Mouse
Unigene: 2681?Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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產品圖片
Sample:
A431(Human) Cell Lysate at 40 ug
Primary: Anti-aPRPSAP2 (bs-19410R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRPSAP2) Polyclonal Antibody, Unconjugated (bs-19410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRPSAP2) Polyclonal Antibody, Unconjugated (bs-19410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRPSAP2) Polyclonal Antibody, Unconjugated (bs-19410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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