二甲基化組蛋白H3K9抗體
產品名稱： 二甲基化組蛋白H3K9抗體
英文名稱： Histone H3 (Di Methyl K9)
產品編號： 3747
產品價格： null
產品產地： 上海
品牌商標： 雅吉
更新時間： null
使用范圍： ELISA IHC-P IHC-F Flow-Cyt ICC IF
上海雅吉生物科技有限公司
- 聯系人 :
- 地址 : 上海市閔行區元江路5500號第1幢5658室
- 郵編 :
- 所在區域 : 上海
- 電話 : 158****3937 點擊查看
- 傳真 : 點擊查看
- 郵箱 : yajikit@163.com
?
| 中文名稱 | 線粒體異檸檬酸脫氫酶抗體 |
| 別????名 | mitochondrial; ICD-M; IDH; IDH2; IDHP_HUMAN; IDP; Isocitrate dehydrogenase [NADP]; Isocitrate dehydrogenase [NADP], mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase.?? |
| 研究領域 | 腫瘤??細胞生物??免疫學??染色質和核信號??轉錄調節因子??激酶和磷酸酶??新陳代謝?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Rat,? (predicted: Mouse,?Dog,?Horse,?) |
| 產品應用 | ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=0.2ug/test?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 51kDa |
| 細胞定位 | 細胞漿? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human IDH2:251-350/452? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit. Function: Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. Subunit: Homodimer. Subcellular Location: Cytoplasm. Peroxisome. DISEASE: Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. SWISS: P48735 Gene ID: 3418 Database links: Entrez Gene: 3418?Human Entrez Gene: 269951?Mouse Entrez Gene: 361596?Rat Omim: 147650?Human SwissProt: P48735?Human SwissProt: P54071?Mouse SwissProt: P56574?Rat Unigene: 596461?Human Unigene: 246432?Mouse Unigene: 290925?Mouse Unigene: 3490?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. IDH2又稱異檸檬酸脫氫酶，他有有兩種類型的酶：以NAD為輔酶的酶（EC1．1．1．41）和NADP的酶（EC1．1．1．42），兩者催化同一反應。在胞漿中以NADP為輔酶，在線粒體內膜以NAD為輔酶。 IDH2在三羧酸循環中正常起作用的是NAD為輔酶的酶，他是提供為ADP激活的變構酶。 |
| 產品圖片 |  Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IDH2) Polyclonal Antibody, Unconjugated (bs-3947R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IDH2 Polyclonal Antibody, Unconjugated(bs-3947R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IDH2 Polyclonal Antibody, Unconjugated(bs-3947R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei  U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IDH2 Antibody(bs-3947R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |