重組人TIGIT蛋白（Recombinant Human TIGIT Protein），GMP級

產品貨號：GMP-TL730

產品描述（Description）

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

分子別名（Synonym）

VSIG9、VSTM3

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列編碼人TIGIT（NCBI Reference Sequence:NP_776160.2）表達帶有Fc標簽在C末端

分子量（Molecular Weight）

重組人TIGIT蛋白 包含351個氨基酸，預測理論分子量為40kD

純度（Purity）

≥95%采用SDS-PAGE凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人TIGIT(His標簽)1ug/mL(100 uL/孔)你能夠結合人CD155（His 標簽)的線性范圍是10-100 ng/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1、TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection Glen M. Chew, Tsuyoshi Fujita, Gabriela M. Webb, Benjamin J. Burwitz, Helen L. Wu, Jason S. Reed, Katherine B. Hammond, Kiera L. Clayton, Naoto Ishii, Mohamed Abdel-Mohsen, Teri Liegler, Brooks I. Mitchell, Frederick M. Hecht, Mario Ostrowski, Cecilia M. Shikuma, Scott G. Hansen, Mark Maurer, Alan J. Korman, Steven G. Deeks, Jonah B. Sacha, Lishomwa C. Ndhlovu PLoS Pathog. 2016 Jan; 12(1): e1005349. Published online 2016 Jan 7. doi: 10.1371/journal.ppat.1005349 

2、TIGIT predominantly regulates the immune response via regulatory T cells Sema Kurtulus, Kaori Sakuishi, Shin-Foong Ngiow, Nicole Joller, Dewar J. Tan, Michele W.L. Teng, Mark J. Smyth, Vijay K. Kuchroo, Ana C. Anderson J Clin Invest. 2015 Nov 2; 125(11): 4053–4062. Published online 2015 Sep 28. doi: 10.1172/JCI81187

  注意：For research and manufacturing use.