description:for research or for further manufacturing use.
sterility:sterile-filtered.
form:liquid.
quality:Drug Master File available.
technique(s):cell culture | insect: suitable.
components:glucose: 6.0 g/L;NaHCO3: 0.35 g/L;HEPES: no;L-glutamine: 1.0 g/L.
Application:EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.