| Catalog Number:??26081 |
| Gene Symbol:??IDH1 |
| Description: ??Anti-IDH1 (R132H) Mouse Monoclonal Antibody |
| Background: ??Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: ??Recombinant protein of human IDH1 which includes the mutation of R132H. |
| Applications: ??ELISA, WB, IHC, IP |
Recommended Dilutions: ???
ELISA: 1:1000-1:5000?
WB: 1:100-1:1000?
IHC: 1:50-1:100 |
| Concentration: ??1 mg/ml |
| Host: ??mouse |
| Format: ??Liquid |
| Clonality: ??Monoclonal |
| Isotype: ??IgG |
| Purity: ??Purified from ascites |
| Storage buffer: |
| Preservative:??no |
| Constituents:??PBS (without Mg?2+?and Ca?2+?), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity:??Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates. |
| Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles |
| ? |
| Western blot:?? |
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Western blot analysis of recombinant IDH1(R132H) and wild-type proteins. Purified His-tagged IDH1 (R132H) protein (Cat.#10199) and wild-type protein (amino acid 1-240) (Cat.#10198) were blotted with anti-IDH1(R132H) mouse antibody (Cat.# 26081)(Left panel) or with anti-IDH1 mouse antibody (Cat. #26136 right panel). |
| Immunofluorescence:?? |
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Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1 (R132H) plasmid (right column), then fixed and stained with anti-IDH1 (R132H) monoclonal antibody (Cat. #26081). |
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Publications:
1.??Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
????Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.??Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
????Nature 513, 110–114 (04 September 2014)
3.??Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
????Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015
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