SUPERDEX 75 PC 3.2/30
產(chǎn)品名稱： SUPERDEX 75 PC 3.2/30
英文名稱： SUPERDEX 75 PC 3.2/30
產(chǎn)品編號： 17-0771-01
產(chǎn)品價格： 0
產(chǎn)品產(chǎn)地： 美國
品牌商標(biāo)： GE
更新時間： null
使用范圍： null
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| 17-0671-01 | MONO Q PC 1.6/5 |
| 17-0672-01 | MONO S PC 1.6/5 |
| 17-0673-01 | SUPEROSE 6 PC 3.2/30 |
| 17-0674-01 | SUPEROSE 12 PC 3.2/30 |
| 17-0686-01 | MINI Q PC 3.2/3 |
| 17-0687-01 | MINI S PC 3.2/3 |
| 17-0771-01 | SUPERDEX 75 PC 3.2/30 |
| 17-1089-01 | SUPERDEX 200 PC 3.2/30 |
| 17-1455-01 | PRECISION COLUMN HOLDER |
| 17-1458-01 | SUPERDEX PEPTIDE PC 3.2/30 |
| 17-1550-01 | COLUMN HOLDER, 10 CM |
| 18-1015-28 | PROTECTIVE CAPS (2/PK) |
| 17-5057-01 | MICRORPC C2-C18 ST 4,6-100 |
| 17-5068-01 | SOURCE 15RPC ST 4.6/100 |
| 17-5116-01 | SOURCE 5RPC ST 4,6-150 |
| 17-5172-01 | SUPEROSE 6 10/300 GL |
| 17-5173-01 | SUPEROSE 12 10/300 GL |
| 17-5174-01 | SUPERDEX 75 10/300 GL |
| 17-5175-01 | SUPERDEX 200 10/300 GL |
| 17-5176-01 | SUPERDEX PEPTIDE 10/300 GL |
| 28-9065-61 | Superdex 200 5_150 GL |
| 28-9205-04 | Superdex 75 5_150 GL |
| 17-0506-01 | PP COLUMN MONO Q HR 16/10 |
| 17-0507-01 | PP COLUMN MONO S HR 16/10 |
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Superdex? 75 / Superdex? 200 Columns
- High-resolution separations of proteins, peptides, and other biomolecules according to size.
- Ideal for the polishing step in a purification procedure.
- Separations in two ranges from Mr 3000 up to 600?000 (globular proteins).
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns
Superdex? 75 / Superdex? 200 Columns
Technical Information
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
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Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
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Superdex columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn? Column Family, that provide excellent reproducibility and durability in purification using a chromatography system.
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| TECHNICAL SPECIFICATIONS | |
| Superdex? | |
| Exclusion limit (Mr)? | |
| Superdex? 75? | 1 × 105 globular protein? |
| Superdex? 200? | 1.3 × 106 globular protein? |
| Separation range (Mr)? | |
| Superdex? 75? | 3000-70?000 globular protein? |
| Superdex? 200? | 10?000-600?000 globular protein? |
| Matrix ? | Spherical composite of cross-linked agarose and dextran? |
| Average particle size? | 13 μm? |
| Chemical stability? | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)? |
| pH stability? | 3-12 (working and long term), 1-14 (short term)? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
| Superdex? 10/300 GL Columns (Tricorn)* | |
| Bed dimensions? | 10 × 300 mm? |
| Recommended sample volume? | 25-250 μl? |
| Bed volume? | 24 ml? |
| Max. pressure? | ? |
| Superdex? 75? | 18 bar (261 psi, 1.8 MPa)? |
| Superdex? 200? | 15 bar (217 psi, 1.5 MPa)? |
| Max. flow rate (H2O at 25°C)? | |
| Superdex? 75? | 1.5 ml/min? |
| Superdex? 200? | 1.0 ml/min? |
| Theoretical plates? | > 30?000 m-1? |
| Superdex? PC 3.2/30 Columns | |
| Bed dimensions? | 3.2 × 300 mm? |
| Bed volume? | 2.4 ml? |
| Recommended sample volume? | 2-25 μl? |
| Max. pressure? | ? |
| Superdex? 75? | 24 bar (348 psi, 2.4 MPa)? |
| Superdex? 200? | 12 bar (174 psi, 1.2 MPa)? |
| Max. flow rate (H2O at 25°C)? | |
| Superdex? 75? | 0.100 ml/min? |
| Superdex? 200? | 0.100 ml/min? |
| Theoretical Plates? | > 30?000 m-1? |
| Superdex? 5/150 column (Tricorn?) | |
| Bed volume? | 3 ml? |
| Bed dimensions? | 5 × 150 mm? |
| Recommended sample volume? | 4 - 50 μl? |
| Recommended flow rate? | 0.15 - 0.6 ml/min ? |
| Max. pressure? | ? |
| Superdex 75? | 1.8 MPa? |
| Superdex 200? | 1.5 MPa? |
| Max. flow rate (H20 at 25°C)? | ? |
| Superdex 75? | 0.7 ml/min? |
| Superdex 200? | 0.8 ml/min? |
| Theoretical plates? | > 25000 m-1? |
| * Columns are not suitable for use with ?KTAprime? plus system.
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Separation of standard proteins on Tricorn? Superdex? 200.
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Separation of monomer and dimer on Tricorn? Superdex? 75.
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Column performance test with a set of standard proteins on Superdex? 75
5/150 GL.
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