Overview

Product name
GST Activity Fluorometric Assay Kit

See all GST products (2) ...


Product overview
The GST Activity Assay Kit provides a simple, fluorescence-based in vitro assay for detecting the GST activity using a fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (ex/em = 380/461 nm) when it reacts with glutathione. GST catalizes the MCB-glutathione reactions and the fluorescence levels are proportionally to the amounts of GST presence in the reaction. Thus, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at -20°C, Stable for 6 months at -20°C



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
Glutathione (lyophilized)
            
Yellow
            
2 vials
        
        

            
GST Activity Fluorometric Assay Kit
            
ab65325
            
1 x 100 kits
        
        

            
GST Assay Buffer
            
NM
            
1 x 10ml
        
        

            
GST Sample Buffer
            
WM
            
1 x 25ml
        
        

            
GST Standard (20 mU/μl)
            
Green
            
1 x 20μl
        
        

            
MCB Substrate
            
Red
            
1 x 200μl
        
    


http://www.abcam.com/GST-Activity-Fluorometric-Assay-Kit-ab65325.html