Product: Anti-BimS/EL/L mAb, clone 14A8
Cat. No.: MC-504 (100 μg)
Specificity:
Recognizes human, mouse and monkey BimL,
BimEL and BimS.
Species Reactivity:
Human, mouse and monkey. Others not tested.
Ig Isotype:
Rat IgG2a
Immunogen:
Recombinant mouse BimS.
Format:
Liquid, 100 μL of Protein G purified monoclonal
antibody at 1 mg/mL in PBS containing 0.02%
sodium azide.
Storage:
Store at 4°C short term, -20°C long term.
Aliquot to avoid freeze/thaw cycles.
Applications:

Western blot

Flow cytometry

Immunoprecipitation

Immunohistochemistry: Paraffin sections

Immunocytochemistry
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Figures: IP/Western blot analysis of Bim expression in
mouse (A) and human (C) cell lines. Bim proteins were
immunoprecipitated from lysates of 107 cells with anti-
BimS/EL/LmAb, clone 14A8 (MC-504) and anti-BimLmAb,, clone
5E5 (MC-505). Western blot analysis was performed with
MC-504 and MC-505 followed by the addition of biotinylated
mouse anti-rat IgG2b antibody and HRP-conjugated mouse
anti-rat IgG2a antibody as the respective secondary
antibodies, and ECL detection. (B) depicts the ability of MC-
504 to immunoprecipitate BimL and BimEL from lysates of
spleen and thymus from normal mice, but not from Bimdeficient
mic