• 經測試應用WBmore details
• 種屬反應性
  與反應:?Mouse, Rat, Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GRID2 aa 1-100 (N terminal). The exact sequence is proprietary.
  Database link:?O43424

• 陽性對照
  • Rat brain, Mouse brain, Human cerebellum, Human fetal brain and HeLa cell lysates.
• 常規說明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存儲溶液Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
• 濃度100 μl 濃度為 1.401 mg/ml?
  
• 純度Protein A purified
• 克隆單克隆
• 克隆編號EPR15422
• 同種型IgG
• 研究領域
  • Neuroscience
  ?
  • Neurotransmitter
  ?
  • Amino Acids
  ?
  • Glutamate

  • Neuroscience
  ?
  • Neurotransmission
  ?
  • Receptors / Channels
  • Ligand-Gated Ion Channels
  ?
  • More Channels

應用

靶標

• 功能Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
• 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.
• 結構域The PDZ-binding motif mediates interaction with GOPC.
• 細胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
• Information by UniProt
• 數據庫鏈接
  • Entrez Gene: 2895?Human
  • Entrez Gene: 14804?Mouse
  • Entrez Gene: 79220?Rat
  • Omim: 602368?Human
  • SwissProt: O43424?Human
  • SwissProt: Q61625?Mouse
  • SwissProt: Q63226?Rat
  • Unigene: 480281?Human

  see all
• 別名
  • delta-2 antibody
  • GluD2 antibody
  • GLUR D2 antibody

  see all

Anti-GRID2 antibody [EPR15422] - N-terminal 圖像

• 

  Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
  All lanes :?Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution
  
  Lane 1 :?Rat brain lysate
  Lane 2 :?Mouse brain lysate
  Lane 3 :?Human cerebellum lysate
  
  Lysates/proteins at 10 μg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size :?113 kDa
  Observed band size :?113 kDa
  
  
  Exposure time :?15 seconds

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
  Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution + Human fetal brain lysate at 10 μg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size :?113 kDa
  Observed band size :?113 kDa
  
  
  Exposure time :?3 minutes

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
  Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution + HeLa cell lysate at 10 μg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size :?113 kDa
  Observed band size :?113 kDa
  
  
  Exposure time :?3 minutes

  Blocking/Dilution buffer: 5% NFDM/TBST.