MONO Q-色譜柱 -耗材-生物在線
上海基星生物科技有限公司
MONO Q

MONO Q

商家詢價

產品名稱: MONO Q

英文名稱: MONO Q

產品編號: 17-5166-01

產品價格: 0

產品產地: USA

品牌商標: 美國GE

更新時間: null

使用范圍: null

上海基星生物科技有限公司
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17-5166-01 MONO Q 5/50 GL
17-5167-01 MONO Q 10/100 GL
17-5168-01 MONO S 5/50 GL
17-5169-01 MONO S 10/100 GL
17-5170-01 MONO P 5/50 GL
17-5171-01 MONO P 5/200 GL
17-5177-01 MINI Q 4.6/50 PE
17-5178-01 MINI S 4.6/50 PE
17-5179-01 MONO Q 4.6/100 PE
17-5180-01 MONO S 4.6/100 PE
17-5181-01 SOURCE 15Q 4.6/100 PE
17-5182-01 SOURCE 15S 4.6/100 PE
17-5186-01 SOURCE 15PHE 4.6/100 PE

MonoBeads? Columns

  • Innovative, monodisperse 10-μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • MonoBeads? available in a choice of three prepacked column formats?Tricorn?, HR, and PC columns.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
  • Compatible with ?KTAdesign?, FPLC? System, and other high-performance LC systems.
  • MonoBeads? are also available as Mono P for chromatofocusing

MonoBeads? Columns

Technical Information


MonoBeads? are prepacked in Tricorn?, HR, and PC column formats. The three Tricorn? (5/50 GL, 10/100 GL, and 4.6/100 PE) columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads? columns (see page 511).

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Tricorn? columns are ideal for the polishing step when high purity is required. HR 16/10 columns, on the other hand, have higher loading capacity than Tricorn? columns. PC 1.6/5 columns are ideal for micropurification of subnanogram to microgram amounts of sample on ?KTA Systems or other high-performance LC systems.

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Customer feedback on Mono Q HR 5/5.

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TECHNICAL SPECIFICATIONS
MonoBeads?
Ion exchanger type? Mono Q?? Strong anion?
? Mono S?? Strong cation?
Ionic capacity? Mono Q?? 0.27-0.37 mmol Cl-/ml?
? Mono S?? 0.12-0.15 mmol H+/ml?
pH stability? ? 2-12 (working), 1-14 (short term)?
Chemical stability? ? Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid.?
Medium? ? Monodisperse polystyrene/divinyl benzene particles?
Particle size? ? 10 μm?
Storage? ? 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S)?
Storage temperature? 4°C to 30°C?
MonoBeads Columns*
Bed volume? PC 1.6/5? 0.1 ml?
? 5/50 GL? 1 ml?
? 4.6/100 PE? 1.7 ml?
? 10/100 GL? 8 ml?
? HR 16/10? 20 ml?
Max. pressure? PC 1.6/5? 50 bar (725 psi, 5 MPa)?
? 5/50 GL, 10/100 GL,
?4.6/100 PE?
40 bar (580 psi, 4 MPa)?
? HR 16/10? 30 bar (435 psi, 3 MPa)?
Recommended flow rate? PC 1.6/5? 0.01-0.4 ml/min?
? 5/50 GL, 4.6/100 PE? 0.5-3.0 ml/min?
? 10/100 GL? 2.0-6.0 ml/min?
? HR 16/10? < 10 ml/min?
* Columns are not suitable for use with ?KTAprime? plus system.

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Click here to view this image in high resolution
Tricorn high-performance columns are prepacked with MonoBeads ion exchange media for high-resolution purification and high capacity.

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Click here to view this image in high resolution
The polishing step on Mono Q 5/50 GL shows different phosphoforms of the purified kinase and very high purity.