Size??100ug
Cat code??200350-F12
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??96kDa
Sensitivity??This antibody detects endogenous levels of ICAM1(C-terminus) and does not cross-react with related proteins.
Immunogen??Purified recombinant human ICAM1(C-terminus) protein fragments expressed in E.coli.
UniProt Number??P05362
GeneBank ID??NM_000201
Subcellular location??Membrane; Single-pass type I membrane protein.
Formulation??Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername??BB 2;BB2;CD 54;CD_antigen=CD54;CD54;Cell surface glycoprotein P3.58;Human rhinovirus receptor;ICAM 1;ICAM-1;ICAM1;ICAM1_HUMAN;Intercellular adhesion molecule 1;Major group rhinovirus receptor;MALA 2;MALA2;MyD 10;MyD10;P3.58
Gene_symbol??BB2; CD54; P3.58
Summary??This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
UniPort summary  Function??ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Application Images :??
Western blot detection of ICAM1 antibody in SW480 and Raji cell lysates using ICAM1 antibody (1:1000 diluted).Predicted band size:96KDa.Observed band size:96KDa.
Immunocytochemistry staining of SW480 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-ICAM1 antibody (dilution 1:200).