Anti-His-tag Mouse Monoclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術有限公司
Anti-His-tag Mouse Monoclonal Antibody

Anti-His-tag Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-His-tag Mouse Monoclonal Antibody

英文名稱： Anti-His-tag Mouse Monoclonal Antibody

產品編號： 28004

產品價格： null

產品產地： 美國

品牌商標： NewEastbio

更新時間： null

使用范圍： ELISA, WB, IHC

武漢紐斯特生物技術有限公司
  • 聯系人 :
  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區域 : 湖北
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  • 郵箱 : neweast3@126.com

His-tag [28004]
[100 μL]		 ?

Catalog Number:??28004
Gene Symbol:??His-tag
Description: ??Anti-His-tag Mouse Monoclonal Antibody
Background: ???Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. It provides a means of specifically purifying or detecting the recombinant protein without a protein-specific antibody or probe.
Immunogen: ??His-tag
Applications: ??WB
Recommended Dilutions: ??
WB 1:1000-1:5000
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??His-tag antibody recognizes expressed His-tagged proteins from vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
?
Western blot:??
Western blot analysis of recombinant His-tagged proteins. Purified His-tagged Vimentin (Cat. #10190, lane 1), RheB (Cat. #10118, lane 2) and Cystatin C (Cat. #10157, lane 3) proteins were blotted with anti-His-tag mouse monoclonal antibody(Cat.# 28004) at 1:1000 dilution(Top panel). Proteins loading were blotted with anti-Vimentin monoclonal antibody (Cat. #26054), anti-RheB monoclonal antibody (Cat. #26015) and anti-Cystatin C monoclonal antibody(Cat. #26044) at 1:1000 dilution, repectivly(Bottom panel).