Product: Anti-CD31 mAb, clone JC/70A
Cat. No.: MC-428 (1 mL)
Synonym:
PECAM-1 (endothelial cell marker)
Background:
CD31 is a glycoprotein expressed on endothelial
cells and in platelets. It is known to be involved
in cell signaling and cell adhesion.
Specificity:
Antibody from clone JC/70A reacts with
endothelial cells in normal tissues and in benign
and malignant proliferations. In cryostat sections
and blood smears, the antibody also stains
megakaryocytes, platelets and occasionally
plasma cells. It reacts weakly with mantle zone
B cells, peripheral T cells and neutrophils.
Antibody to CD31 is of value in the study of
benign and malignant vascular tumors. Staining
for CD31 has also been used to measure
angiogenesis, which reportedly predicts tumor
recurrence.
Species Reactivity:
Human. Does NOT react with rat or pig. Other
species not tested.
Ig Isotype:
Mouse IgG1/k
Immunogen:
Membrane preparation of a spleen from a
patient with hairy cell leukemia.
Molecular Weight of Antigen:
100 kDa (endothelial cells) and 130 kDa
(platelets)
Positive Control:
Placenta
Cellular Localization:
Cell membrane
Epitope:
Not Determined.
Format:
1 mL of monoclonal antibody in tissue culture
supernatant with 0.09% sodium azide.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Immunohistochemistry: (Formalin/paraffin),
use at a 1:25 dilution for 30 minutes at room
temperature (RT). Staining of formalin-fixed
tissue requires boiling tissue sections in 1 mM
EDTA, pH 8.0, for 10-20 minutes followed by
cooling at RT for 20 minutes.

Immunofluorescence
Note: Not suitable for Western blots.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product