PROTEIN G SEPHAROSE 4 FF
產(chǎn)品名稱(chēng)： PROTEIN G SEPHAROSE 4 FF
英文名稱(chēng)： PROTEIN G SEPHAROSE 4 FF
產(chǎn)品編號(hào)： 17061801
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 美國(guó)
品牌商標(biāo)： GE
更新時(shí)間： null
使用范圍： null
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| 17-0618-01 | PROTEIN G SEPHAROSE 4 FF, 5 ML |
| 17-0618-02 | PROTEIN G SEPHAROSE 4 FF, 25 M |
| 17-0618-05 | PROTEIN G SEPHAROSE 4FF 200 ML |
| 17-0640-01 | ACT THIOL SEPHAROSE 4B 15 G |
| 17-0690-01 | LYSINE-SEPHAROSE 4B?15 G |
| 17-0700-01 | 2,5 ADP-SEPHAROSE 4B?? 5 G |
| 17-0756-01 | GLUTATHIONE SEPHAROSE 4B?10 ML |
| 17-0756-04 | GLUTATHIONE SEPHAROSE 4B, 300ML |
| 17-0756-05 | GLUTATHIONE SEPHAROSE 4B, 100M |
| 17-0780-01 | PROTEIN A-SEPHAROSE CL-4B 1.5G |
| 17-0820-01 | CNBR ACTIVATED SEPHAROSE 6MB |
| 17-0885-01 | GAMMABIND G SEPHAROSE, 5 ML |
| 17-0885-02 | GAMMABIND G SEPHAROSE, 25 ML |
| 17-0885-04 | GAMMABIND G SEPHAROSE, 250 ML |
| 17-0886-01 | GAMMABIND PLUS SEPHAROSE, 5 ML |
| 17-0886-02 | GAMMABIND PLUS SEPHAROSE, 25 M |
| 17-0886-04 | GAMMABIND PLUS SEPHAROSE, 500ML |
| 17-0906-01 | NHS ACT SEPHAROSE 4 FF 25 ML |
| 17-0920-06 | IMAC SEPHAROSE HP, 25 ML |
| 17-0920-07 | IMAC SEPHAROSE HP, 100 ML |
| 17-0921-07 | IMAC SEPHAROSE 6 FF, 25 ML |
| 17-0921-08 | IMAC SEPHAROSE 6 FF, 100 ML |
| 17-0948-01 | BLUE SEPHAROSE 6 FAST FLOW, 50ML |
| 17-0956-01 | GELATIN-SEPHAROSE 4B,?25 ML |
| 17-0963-02 | PROTEIN A SEPHAROSE CL-4B 500M |
| 17-0963-03 | PROTEIN A SEPHAROSE CL-4B, 25 |
| 17-0969-01 | IGG SEPHAROSE 6 FAST FLOW?10 |
Protein G Sepharose? 4 Fast Flow
- High capacity, high flow rate fractionation of monoclonal antibodies at both laboratory and process scales, without binding albumin.
- Rapid processing of large volumes, recoveries of 70-90%.
- Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse
Protein G Sepharose? 4 Fast Flow
Technical Information
| TECHNICAL SPECIFICATIONS | |
| Ligand? | Recombinant protein G lacking albumin-binding region? |
| Ligand coupling method? | Cyanogen bromide activation? |
| No.of IgG binding? | 2 sites per ligand? |
| MW of ligand? | ~17 000? |
| pI of ligand? | 4.1? |
| Degree of substitution? | ~2 mg Protein G/ml drained medium? |
| Number of IgG binding sites?per ligand? | 2? |
| Matrix? | Highly cross-linked agarose, 4%? |
| Binding capacity? | > 20 mg human IgG/ml medium? |
| Average particle size? | 90 μm? |
| Ligand density? | ? 2 mg Protein G/ml medium? |
| Chemical stability? | Stable to all commonly used aqueous buffers - 1 M acetic acid, 1% SDS, and 6 M guanidine HCl (tested at 37°C for 7 days), as well as, 0.1 M glycine NaOH pH 11, 1 M HC1, and 8 M urea (stable for at least 2 h at room temperature)? |
| pH stability? | 3-9 (working), 2*-10 (short term)? |
| Physical stability? | Negligible volume variation due to changes in pH or ionic strength? |
| Sanitization? | Do not autoclave.Wash the column with 70% ethanol.? |
| Antimicrobial agent? | 20% ethanol? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 8°C? |
| * ? pH below 3 is sometimes required to elute strongly bound IgG species. However, ?protein ligands may hydrolyze at very low pH.
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Purification of human monoclonal antibody using Protein G Sepharose? 4 Fast Flow (1).
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Reference
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- Jungbauer, A. et al. Comparison of protein A, protein G and copolymerised hydroxyapatite for the purification of human monoclonal antibodies. J. Chromatogr. 476, 257 (1989).