抗人RSPO1 / R-spondin-1兔單抗
產(chǎn)品名稱(chēng)： 抗人RSPO1 / R-spondin-1兔單抗
英文名稱(chēng)： RSPO1 / R-spondin-1 Antibody
產(chǎn)品編號(hào)： 11083-R106
產(chǎn)品價(jià)格： null
產(chǎn)品產(chǎn)地： 北京
品牌商標(biāo)： 義翹神州(SinoBiologi
更新時(shí)間： null
使用范圍： ELISA
北京義翹神州科技有限公司
- 聯(lián)系人 :
- 地址 : 北京亦莊東區(qū)科創(chuàng)十街18號(hào)院9號(hào)樓306室
- 郵編 : 100176
- 所在區(qū)域 : 北京
- 電話(huà) : 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : marketing@sinobiological.cn
官方網(wǎng)址：http://www.sinobiological.cn/RSPO1-Antibody-g-5153.html
R-spondin-1 / RSPO1 Antibody
| ? |  | Order or Inquire?for RSPO1 Antibody product |  |
| ? | | Detection limit is 0.00975 ng/well in ELISA |
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R-spondin-1 / RSPO1抗體產(chǎn)品信息
| 免疫原 : | Recombinant human RSPO1 protein ( Catalog#11083-H08H ) |
| Antibody Type : | Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform ) |
| 克隆號(hào) : | 106 |
| 抗體宿主 : | Rabbit IgG |
| 緩沖液 : | 0.2 μm filtered solution in PBS with 5% trehalose |
| 制備方法 : | This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human RSPO1 / R-spondin-1 ( rh RSPO1 ; Catalog#11083-H08H ; NP_001033722.1 ; Met 1 - Ala 263 ). |
R-spondin-1 / RSPO1 Antibody Usage Guide
| 特異性 : | Human RSPO1 / R-spondin-1 |
| ? | No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. |
| Direct ELISA : | This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human RSPO1. The detection limit for human RSPO1 is approximately?0.00975 ng/well |
| 儲(chǔ)存方法 : | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.?Avoid repeated freeze-thaw cycles. |
R-spondin-1 / RSPO1 Antibody Related Products & Topics
R-spondin-1 / RSPO1 Antibody 相關(guān)領(lǐng)域:
Proteins:
Antibodies:
R-spondin-1 / RSPO1 抗體背景綜述
R-Spondin 1 (Roof plate-specific Spondin 1), also known as RSPO1 and Cristin 3, is a secreted protein which belongs to the R-Spondin family. The R-Spondin (RSPO) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. R-Spondin1 regulates cellular responsiveness to Wnt ligands by modulating the cell-surface levels of the coreceptor LRP6. R-Spondin1 activity critically depends on the presence of canonical Wnt ligands and LRP6. R-Spondin1 does not directly activate LRP6, it interferes with DKK1/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 levels on the cell surface. Mature human R-Spondin1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. Human R-Spondin1 shares 89%, 87%, 92%, 91%, 91%, and 89% aa identity with mouse, rat, equine, canine, caprine and bovine R-Spondin1, respectively. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY.
參考文獻(xiàn)
- Parma, P. et al., 2006, Nature genetics.?38?(11): 1304-9.
- Capel, B., 2006, Nature genetics. 38 (11) :1233-4.
- Binnerts, ME.?et al., 2007, Proc Natl Acad Sci.?104?(37): 14700-5.
- Wilhelm, D., 2007,?Bioessays.??29?(4): 314-8.
- Zhao, J. et al., 2009, Proc Natl Acad Sci. 106 (7) :2331-6.
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