人視網膜色素上皮細胞（ARPE-19）
產品名稱： 人視網膜色素上皮細胞（ARPE-19）
英文名稱： ARPE19
產品編號： TCH-C127
產品價格： 0
產品產地： 蘇州
品牌商標： HyCyte™
更新時間： null
使用范圍： null
- 聯系人 :
- 地址 : 太倉市開發區
- 郵編 : 215300
- 所在區域 : 江蘇
- 電話 : 131****7797 點擊查看
- 傳真 : 點擊查看
- 郵箱 : hfx@dingbio.com
該細胞系源自于一名19歲車禍罹難的健康男性的視網膜組織，由AmyAotaki-Keen建系于1986年。該細胞系表達視網膜色素細胞特有的分子標記如胞內視黃醛結合蛋白和PRE-65。
細胞名稱：人視網膜色素上皮細胞（STR鑒定正確）
細胞簡稱：ARPE-19
產品貨號：TCH-C127
種屬來源：人
組織來源：視網膜
疾病特征：/
細胞形態：上皮細胞樣
生長特性：貼壁生長
培養體系：DF-12+10%FBS+1%P/S
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
參考文獻：
"Hazim RA, et al. Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide. Exp Eye Res 179: 18-24, 2018. PubMed: 30336127
Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076
Maidji E, et al. Accessory human cytomegalovirus glycoprotein US9 in the unique short component of the viral genome promotes cell-to-cell transmission of virus in polarized epithelial cells. J. Virol. 70: 8402-8410, 1996. PubMed: 8970961
Holtkamp GM, et al. Polarized secretion of IL-6 and IL-8 by human retinal pigment epithelial cells. Clin. Exp. Immunol. 112: 34-43, 1998. PubMed: 9566787
Finnemann SC, et al. Phagocytosis of rod outer segments by retinal pigment epithelial cells requires alpha(v)beta5 integrin for binding but not for internalization. Proc. Natl. Acad. Sci. USA 94: 12932-12937, 1997. PubMed: 9371778
Handa JT, et al. The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells. Exp. Eye Res. 66: 411-419, 1998. PubMed: 9593635
Dunn KC, et al. Use of the ARPE-19 cell line as a model of RPE polarity: basolateral secretion of FGF5.. Invest. Ophthalmol. Vis. Sci. 39: 2744-2749, 1998. PubMed: 9856785
Tugizov S, et al. An acidic cluster in the cytosolic domain of human cytomegalovirus glycoprotein B is a signal for endocytosis from the plasma membrane. J. Virol. 73: 8677-8688, 1999. PubMed: 10482621
Orten DJ, et al. Analysis of DNA elements that modulate myosin VIIA expression in humans. Hum. Mutat. 14: 354, 1999. PubMed: 10502787
Rajan PD, et al. Expression of the extraneuronal monoamine transporter in RPE and neural retina. Curr. Eye Res. 20: 195-204, 2000. PubMed: 10694895
Janssen JJ, et al. Retinoic acid delays transcription of human retinal pigment neuroepithelium marker genes in ARPE-19 cells. Neuroreport 11: 1571-1579, 2000. PubMed: 10841379
Udono T, et al. Adrenomedullin in cultured human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1962-1970, 2000. PubMed: 10845623
Sparrow JR, et al. The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1981-1989, 2000. PubMed: 10845625"