促甲狀腺素受體抗體（N端）
產品名稱： 促甲狀腺素受體抗體（N端）
英文名稱： TSHR (NT)
產品編號： bs-0003R
產品價格： null
產品產地： 北京
品牌商標： 博奧森
更新時間： 2025-12-25T09:37:19
使用范圍： WB=1:100-500,ELISA=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,
- 聯系人 : 索萊寶-龔思雨
- 地址 : 北京市北京經濟技術開發區環科中路16號26幢3層301
- 郵編 :
- 所在區域 : 北京
- 電話 : 181****6239 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 3193328036@qq.com
- 二維碼 : 點擊查看
北京索萊寶科技有限公司 ?張凈花 ?010-56371206 ?13426459985
| 產品編號 | bs-0003R |
| 英文名稱 | TSHR (NT) |
| 中文名稱 | 促甲狀腺素受體抗體（N端） |
| 別????名 | hTSHR I; hTSHRI; LGR 3; LGR3; MGC75129; Thyroid adenoma hyperfunctioning; Thyroid carcinoma with thyrotoxicosis; Thyroid Stimulating Hormone Receptor; Thyrotropin Receptor; Thyrotropin receptor I; TSH R; TSHR; TSHR_HUMAN. |
| 規格價格 | 0.1ml/600元?購買????0.2ml/1000元?購買???? |
| 說?明?書 | 0.1ml??0.2ml?? |
| 研究領域 | 免疫學??神經生物學??信號轉導??生長因子和激素??細胞膜受體?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,?Rat,?Dog,?Cow,?Sheep,? |
| 產品應用 | WB=1:100-500?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?ICC=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復）? not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 86kDa |
| 細胞定位 | 細胞膜? |
| 性????狀 | Lyophilized or Liquid |
| 濃????度 | 1mg/1ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human TSHR |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產品介紹
background:
The glycoprotein hormone receptor family consists of the luteinizing hormone receptor, the follicle-stimulating hormone receptor, and the thyroid stimulating hormone(TSH) receptor. TSH, which is released from the pituitary gland, binds to the TSH receptor on thyroid cells to control size and function of the thyroid gland (De Felice et al. 2004). The TSH receptor signals through Gs to elevate intracellular cAMP in the thyroid gland, which regulates iodide uptake, and transcription of thyroglobulin (Tg), thyroid peroxidase (TPO), and sodium-iodide symporter. The TSH receptor also signals Gq and phospholipase C to regulat iodide efflux, H2O2 production, and thyroglobulin iodination. Autoimmunity to the TSH receptor causes hyperthyroidism (Graves disease) or hypothyroidism (Hashimoto thyroiditis) when the autoantibodies function as agonists or antagonists, respectively, at the TSH receptor (Rapoport and McLachlan, 2001; Davies et al., 2002). Millipore’s cloned human TSH receptor-expressing cell line is made in the Chem-10 host, which supports high levels of recombinant TSH receptor expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between TSH and its ligands.
Function:
Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).?
Subunit:
Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Expressed in the thyroid.
DISEASE:
Note=Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
Similarity:
Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.?
Contains 7 LRR (leucine-rich) repeats.
Gene ID:
7253
Database links:
Entrez Gene: 7253?Human
Omim: 603372?Human
SwissProt: P16473?Human
Unigene: 123078?Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.?
TSHR是垂體前葉嗜堿細胞分泌的一種糖蛋白，直接作用于甲狀腺，并能影響其結構功能。該抗體主要用于垂體腫瘤功能性分類的研究。
產品圖片
Cell: HepG2(1% Paraformaldehyde fixed for 30 minutes).
Concentration primary antibody1:50,Secondary antibody1:25?
Incubation primary antibody: 1 hour,Secondary antibody 40 minutes.?
Host/Blank: HepG2.?
Flow cytometric analysis of Rabbit Anti-TSHR (NT)antibody (bs-0003R)(green) compared with control in the absence of primary antibody (blue) followed by HepG2.
Secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC).
Cell: Raji(2% Paraformaldehyde fixed for 10 minutes).
Concentration primary antibody1:50,Secondary antibody1:25?
Incubation primary antibody: 1 hour,Secondary antibody 40 minutes.?
Host/Blank: Raji.?
Flow cytometric analysis of Rabbit Anti-TSHR (NT)antibody (bs-0003R)(green) compared with control in the absence of primary antibody (blue) followed by Raji.
Secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC).
Cell: Raji(2% Paraformaldehyde fixed for 10 minutes).
Concentration:1:50.?
Incubation: 1 hour.?
Host/Blank: Raji.?
Flow cytometric analysis of Rabbit Anti-TSHR (NT)/PE Conjugated antibody (bs-0003R-PE)(green) compared with control in the absence of primary antibody (blue) followed by Raji.
Cell: HepG2(1% Paraformaldehyde fixed for 30 minutes).
Concentration:1:50.?
Incubation: 1 hour.?
Host/Blank: HepG2.?
Flow cytometric analysis of Rabbit Anti-TSHR (NT)/AF488 Conjugated antibody (bs-0003R-AF488)(green) compared with control in the absence of primary antibody (blue) followed by HepG2.
?