重組人GITR Ligand蛋白（Recombinant Human GITR Ligand Protein），GMP級

產品貨號：GMP-TL721

產品描述（Description）

Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18 / GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.

分子別名（Synonym）

TNFSF18、AITRL、TL6、hGITRL

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列編碼人GITR Ligand（GenBank: AAD22634.1）表達帶有Fc標簽在C末端

分子量（Molecular Weight）

重組人GITR Ligand蛋白 包含359個氨基酸，預測理論分子量為41.2 kD

純度（Purity）

≥95%采用SDS-PAGE凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 GITR(His 標簽)1ug/mL (100ul/孔)能夠結合 GITR Ligand( Fc 標簽) 的線性范圍是 5-20ng/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1、Murine B Cell Development and Antibody Responses to Model Antigens Are Not Impaired in the Absence of the TNF Receptor GITR Lenka Sinik Teodorovic, Carlo Riccardi, Raul M. Torres, Roberta Pelanda PLoS One. 2012; 7(2): e31632. Published online 2012 Feb 6. doi: 10.1371/journal.pone.0031632 

2、 Pharmacological modulation of GITRL/GITR system: therapeutic perspectivesGiuseppe Nocentini, Simona Ronchetti, Maria Grazia Petrillo, Carlo Riccardi Br J Pharmacol. 2012 Apr; 165(7): 2089–2099. doi:10.1111/j.1476-5381.2011.01753.x 

3、 Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial gliomaMira A. Patel, Jennifer E. Kim, Debebe Theodros, Ada Tam, Esteban Velarde, Christina M. Kochel, Brian Francica, Thomas R. Nirschl, Ali Ghasemzadeh, Dimitrios Mathios, Sarah Harris-Bookman, Christopher C. Jackson, Christina Jackson, Xiaobu Ye, Phuoc T. Tran, Betty Tyler, Vladimir Coric, Mark Selby, Henry Brem, Charles G. Drake, Drew M. Pardoll, Michael Lim J Immunother Cancer. 2016; 4: 28. Published online 2016 May 17. doi: 10.1186/s40425-016-0132-2 Correction in: J Immunother Cancer. 2016; 4: 74

  注意：For research and manufacturing use.