GSTRAP 4B-色譜柱 -耗材-生物在線

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上海基星生物科技有限公司
GSTRAP 4B

GSTRAP 4B

商家詢價

產品名稱： GSTRAP 4B

英文名稱： GSTRAP 4B

產品編號： 28-4017-45

產品價格： 0

產品產地： 美國

品牌商標： GE

更新時間： null

使用范圍： null

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28-4017-45 GSTRAP 4B, 5 X 1 ML
28-4017-46 GSTRAP 4B, 100 X 1 ML
28-4017-47 GSTRAP 4B, 1 X 5 ML
28-4017-48 GSTRAP 4B, 5 X 5 ML
28-4017-49 GSTRAP 4B, 100 X 5 ML
28-4058-44 HiTrap Capto Adhere 5x1ml
28-4058-46 5x5 ml Hitrap Capto adhere
28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML
28-4110-01 HiTrap Butyl HP,?5x1ml
28-4110-05 HiTrap Butyl HP, 5 x 5ml
28-4110-07 HiTrap HIC Selection Kit,?7x1
28-9075-46 StrepTrap HP 5 x 1mL
28-9075-47 StrepTrap HP 1 x 5mL
28-9075-48 StrepTrap HP 5 x 5mL
28-9165-37 HiTrap Capto DEAE, 5X1ML
28-9165-40 HiTrap Capto DEAE, 5X5ML
28-9187-78 MBPTrap 5 x 1mL
28-9187-79 MBPTrap 1 x 5 mL
28-9187-80 MBPTrap 5 x 5mL
28-9343-88 HiTrap Capto IEX Selection Kit
28-9520-85 HiTrap Con A 4B 5 x 1 ml
28-9520-96 HiTrap Con A 4B 5 x 5 ml

Glutathione Sepharose? 4B Media/GSTrap 4B Columns - Easy Purification

  • Fast and simple purification of GST*-tagged proteins.
  • GSTrap? 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose? 4B.
  • GSTrap? 4B are designed to be used with a syringe, pump, or chromatography system, such as ?KTAdesign

Glutathione Sepharose? 4B Media/GSTrap 4B Columns - Easy Purification

Technical Information


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GSTrap 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose 4B for fast purification of GST-tagged proteins and scale-up.

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GSTrap? 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.

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For an introduction to Glutathione S-transferase (GST) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System.

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Columns: Capture ? GSTrap 4B 1?ml
Polishing ? HiLoad 16/60 Superdex 200 pg
Sample: Cell extract from E. coli expressing GST-hippocalcin (Mr 45?000)
after lysis with CelLytic Express
AC binding/wash buffer: 10?mM sodium phosphate, 140?mM, pH?7.4
AC elution buffer: 50?mM Tris-HCl, 10?mM reduced glutathione, pH?8.0
GF buffer: 50?mM phosphate buffer, 150?mM NaCl, pH?7.2
System: ?KTAprime plus

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Click here to view this image in high resolution
1 Low molecular weight markers
2 Start material (diluted 1:20)
3 Eluted pool GSTrap 4B
4 Eluted pool GSTrap 4B (diluted 1:50)
5 Peak 1, HiLoad 16/60 Superdex 200?pg
6 Peak 2, HiLoad 16/60 Superdex 200?pg

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TECHNICAL SPECIFICATIONS
Ligand? Glutathione?
Ligand coupling method? Epoxy activation?
Dynamic binding capacity*? > 5 mg horse liver GST/ml medium?
Matrix? Agarose, 4%?
Average particle size? 90 μm?
pH stability? 4-13?
Storage? 20% ethanol?
Storage temperature? 4°C to 30°C?
HiTrap prepacked columns
Column volume? 1 ml and 5 ml?
Column dimensions? 0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)
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Recommended flow rate?? 0.2-1 ml/min (1 ml); 0.5-5 ml/min (5 ml)
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Max. pressure?? 3 bar (0.3 MPa)?
* Binding of GST to glutathione is flow-rate dependent and lower flow rates often iincrease the binding capacity. This is an important consideration, especially during sample loading and elution.
? H2O at 25°C.

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http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=38863