人轉(zhuǎn)錄誤調(diào)節(jié)在實(shí)體腫瘤中的定量PCR芯片試劑盒
產(chǎn)品名稱： 人轉(zhuǎn)錄誤調(diào)節(jié)在實(shí)體腫瘤中的定量PCR芯片試劑盒
英文名稱： GQH-TMS
產(chǎn)品編號(hào)： GK031
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 美國
品牌商標(biāo)： sciecnell
更新時(shí)間： 2025-11-18T13:59:09
使用范圍： null
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?人轉(zhuǎn)錄誤調(diào)節(jié)在實(shí)體腫瘤中的定量PCR芯片試劑盒描述：
? ? ? ? ? ScienCells GeneQuery? Human Transcriptional Misregulation in Solid Tumors qPCR Array Kit (GQH-TMS) is designed to facilitate gene expression profiling of 88 key genes involved in six types of solid tumor development (prostate cancer, kidney cancer, thyroid cancer, neuroblastoma, carcinoid, and sarcomas). Brief examples of how included genes may be grouped according to function and tumor types are shown below: ? ??
- Cell cycle regulation:?CDK9, CDKN1A, CDKN1B
- Apoptosis and proliferation:?TP53, RXRs, PPARG, MDM2
- Cellular invasion:?MMP9, SPINT1, IL1R2, PLAU, PLAT, ZEB1
- Cell survival:?BMI1, MYC, IGF1, MLF1
- Mutated transcription factors identified in
- prostate cancer:?ERG, ETV1, ETV4, ELK4
- kidney cancer:?TFE3
- thyroid cancer:?PAX8
- neuroblastoma:?MYCN
- sarcomas:?ATF1, FEV, WTI, DDIT3
GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.?
GeneQuery? Human Transcriptional Misregulation in Solid Tumors qPCR Array Plate Layout*
(8 controls?in Bold and Italic)
?
|
? |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AFF1 |
CCNT2 |
DDX5 |
FUS |
HIST1H3D |
HIST2H3D |
MAX |
MYC |
PAX8 |
SLC45A3 |
TFE3 |
β-actin |
|
B |
ARNT2 |
CDK9 |
ELK4 |
GOLPH3 |
HIST1H3E |
HIST3H3 |
MDM2 |
MYCN |
PLAT |
SMAD1 |
TGFBR2 |
GAPDH |
|
C |
ASPSCR1 |
CDKN1A |
ERG |
GOLPH3L |
HIST1H3F |
IGF1 |
MEN1 |
NFKB1 |
PLAU |
SP1 |
TMPRSS2 |
LDHA |
|
D |
ATF1 |
CDKN1B |
ETV1 |
H3F3B |
HIST1H3G |
IGF1R |
MET |
NFKBIZ |
PPARG |
SPINT1 |
TP53 |
NONO |
|
E |
ATM |
CEBPB |
ETV4 |
H3F3C |
HIST1H3H |
IL1R2 |
MLF1 |
NGFR |
PRCC |
SS18 |
TSPAN7 |
PPIH |
|
F |
BAIAP3 |
COMMD3 |
EWSR1 |
HIST1H3A |
HIST1H3I |
IL2RB |
MLLT1 |
NTRK1 |
RXRA |
SSX2 |
WT1 |
GDC |
|
G |
BMI1 |
CXCL8 |
FEV |
HIST1H3B |
HIST1H3J |
IL6 |
MLLT3 |
NUPR1 |
RXRB |
SSX2B |
ZBTB17 |
PPC |
|
H |
CCNT1 |
DDIT3 |
FOXO1 |
HIST1H3C |
HIST2H3A |
KMT2A |
MMP9 |
PAX7 |
RXRG |
TAF15 |
ZEB1 |
NTC |
?
* gene selection may be updated based on new research and development
| Catalog No. | GK031 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-TMS |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-TMS is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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