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Method of Production? : Transient transfection in 293 cell culture????

Method of Purification??: Protein A affinity chromatography and SEC

Formulation:???PBS pH7.4

Concentration:???2.50 mg/mL

Eedotoxin：＜1EU/ug

0.2μm Filtration：Yes

表達宿主：Human Cells

純度:> 95 % as determined by SDS-PAGE.

內毒素：<1.0 EU per μg protein as determined by the LAL method.

蛋白構建：

A DNA sequence encoding the receptor binding domain (RBD，319-541aa) of 2019-nCoV spike was expressed with the Fc region of mouse IgG1 at the C-terminus.

GIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTN

融合Fc標簽氨基酸序列：

PRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQV

YTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK

分子量：

The recombinant receptor binding domain (RBD) of 2019-nCoV spike consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.