SPECIFICATION?SHEET

Catalog?#:????L1C00205????????????????????Lot?#:C080401

Description:?????MAb?to?Carcinoembryonic?Ag
????Monoclonal?Antibody?to?Carcinoembryonic?Antigen?(CEA)

Specificity:????Reacts?with?a?protein?determinant?belonging?to?the?"Gold"?epitope?group5(3).

Clone:????ML01C05

Host?Animal:????Mouse.??Hybridization?of?X63-Ag8?myeloma?cells?with?spleen?cells?from?Balb/c?mice.????

Isotype:?????IgG1
????
Source:?????Mouse?Ascites?

Immunogen:???????CEA?isolated?from?colorectal?carcinomas?by?PCA?extraction.

Format:????Purified,?Liquid

Purification:??????Protein?A?chromatography?followed?by?gel?filtration.

Concentration:??????????2mg/ml?(OD280nm,?E0.1%?=?1.4)

Affinity?Constant:??????5?x?10-10?mol/l

Buffer:????0.01M??PH7.4??PBS

Preservative:?????None

Applications:?????Determination?of?CEA.??Each?laboratory?should?determine?an?optimum?working?titer?for?use?in?its?particular?application.??Other?applications?have?not?been?tested?but?use?in?such?assays?should?not?necessarily?be?excluded.
Recommended?antibody?pair?for?immunoassay:
????Capture????Detection
????L1C00205????L1C00202??

QC?Information:?????IgG?>98%?of?total?peak?area?(Superdex?200?gel?chromatography?on?AktaFPLC)

Storage:????Short-term?(up?to?2?months)?store?at?2?months)?store?at?<–40°C.