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Animal-Free PeproGrow Low Protein hESC Embryonic Stem Cell Media

Catalog Number:
AF-LP-hESC
Description:
PeproGrow Animal-Free Low Protein hESC Medium is an animal component-free, serum- and phenol red-free medium of a complete, chemically-defined formulation designed for feeder-free maintenance and expansion of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). This medium formulation is best used with PeproTech’s Animal-Free Human Vitronectin Matrix as a surface-coating reagent; however, it can also be used with Corning Matrigel? or Synthemax? II-SC. This medium is intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (Nanog+/Lin28+/Tra-1-60+ or SSEA4+/Oct4+), and demonstrates less than 15% spontaneous differentiation as indicated by immunofluorescent staining. Similar to the PeproGrow hESC Medium (Catalog Number BM-hESC), this proprietary formulation includes relevant growth factors, such as FGF2 (FGF-basic), but does not contain the insulin found in the majority of other hESC/iPSC media currently available on the market. This medium does not contain albumin so researchers may need to adjust laboratory techniques that were developed for albumin-containing media. PeproGrow Animal-Free Low Protein hESC Medium, which was designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University, is supplied as a 100mL, or 500mL, bottle of basal medium and a separate, lyophilized growth factor component. Additional companion products, such as the Animal-Free Human Vitronectin Matrix and Buffer Kit, and the Cell Passaging/Non-Enzymatic Detachment Buffer, are available separately.





Immunostaining of iPSCs cultured in PeproGrow Animal-Free Low Protein hESC media.



Manufactured using all Animal-Free reagents.
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Research Interests:


    

        

            

            
Cell Culture
            

            
FGF Superfamily
        
        

            

            
Stem Cells & Differentiation
            
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Country of Origin:
USA