• 特異性This antibody detects endogenous level of total 14-3-3 epsilon protein.
• 經測試應用ICC/IF,?IHC-P,?WBmore details
• 種屬反應性
  與反應:?Mouse, Rat, Human, African Green Monkey
• 免疫原

  Recombinant full length protein corresponding to Human 14-3-3 epsilon aa 1-255.
  Sequence:

  MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYK NVIGARRASW RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICC DILDVLDKHLIPAANTGESKVF YYKMKGDYHRYLAEFATGNDRKEAAE NSLVAYKAASDIAMTELPPTHPIRLGLALNFSVF YYEILNSPDRACRL AKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGE EQ NKEALQDVEDENQ

  
  Database link:?P62258

  ?

  Run BLAST with?Run BLAST with?
• 陽性對照
  • T47D, Jurkat, PC12, COS1, COS7, NIH3T3 and SW480 cell extracts.

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存儲溶液pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
  
  without Mg2+ and Ca2+
• 濃度100 μl 濃度為 1 mg/ml?
  
• 純度Immunogen affinity purified
• 克隆多克隆
• 同種型IgG
• 研究領域
  • Neuroscience
  ?
  • Neurology process
  ?
  • Neural Signal Transduction

  • Signal Transduction
  ?
  • Adapters
  ?
  • Cytoplasmic

  • Neuroscience
  ?
  • Cell Type Marker
  ?
  • Neuron marker
  ?
  • Synapse marker

  • Stem Cells
  ?
  • Signaling Pathways
  ?
  • Wnt
  ?
  • 14-3-3

應用

靶標

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• 序列相似性Belongs to the 14-3-3 family.
• 細胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Information by UniProt
• 數據庫鏈接
  • Entrez Gene: 7531?Human
  • Entrez Gene: 22627?Mouse
  • Entrez Gene: 29753?Rat
  • Omim: 605066?Human
  • SwissProt: P62258?Human
  • SwissProt: P62259?Mouse
  • SwissProt: P62260?Rat
  • Unigene: 513851?Human

  see all
• 別名
  • 14 3 3 E antibody
  • 14 3 3 epsilon antibody
  • 14 3 3E antibody

  see all

Anti-14-3-3 epsilon antibody 圖像

• 

  Western blot - Anti-14-3-3 epsilon antibody (ab196891)
  All lanes :?Anti-14-3-3 epsilon antibody (ab196891) at 1/500 dilution
  
  Lane 1 :?T47D cell extract
  Lane 2 :?Jurkat cell extract
  Lane 3 :?PC12 cell extract
  Lane 4 :?COS1 cell extract
  Lane 5 :?COS7 cell extract
  Lane 6 :?NIH 3T3 cell extract
  Lane 7 :?SW480 cell extract
  
  
  Predicted band size :?29 kDa