BRAF(V600E) 小鼠單抗
產品名稱： BRAF(V600E) 小鼠單抗
英文名稱： Anti-BRaf(V600E) Mouse Monoclonal Antibody
產品編號： 26039
產品價格： null
產品產地： 美國
品牌商標： Neweast Biosciences
更新時間： null
使用范圍： ELISA, WB, IF, IHC
- 聯系人 :
- 地址 : 武漢東湖新技術開發區高新大道666號武漢國家生物產業基地項目（生物創新園）B.C.D區研發樓B3-3棟3樓
- 郵編 :
- 所在區域 : 湖北
- 電話 : 181****2833 點擊查看
- 傳真 : 點擊查看
- 郵箱 : nebio0923@163.com
| 背景介紹: ??B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers |
| 免疫原: ??A synthetic peptide from the internal region of BRaf(V600E), human origin |
| 經過測試的應用: ??ELISA, WB, IHC, IP |
| 推薦稀釋比: ?? ELISA: 1: 100-1: 5000 WB: 1: 100-1: 2000 IHC: 1: 100-1: 1000 |
| 濃度: ??1 mg/ml |
| 宿主: ??Mouse |
| 形式: ??Liquid |
| 克隆性: ??Monoclonal |
| 亞型: ??IgG2b |
| 純度: ??Purified from ascites |
| 防腐劑:???No |
| 成分:???PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 種屬反應性: ??Recognizes?B-Raf(V600E), but not wild type B-Raf proteins?from vertebrates. |
| 儲存條件: ??Store at -20°C. Avoid repeated freezing and thawing |
蛋白質印跡: ??
Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .
免疫熒光: ??
Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).
Immunohistochemistry: ??
B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.Allele specific PCR validated to be Negative for BRAF V600E.