Product: Anti-MVP/LRP mAb, clone 1014
Cat. No.: MC-895 (100 μg)
Specificity:
This antibody is specific for a 104 kDa Major
Vault Protein (MVP), also described as the lung
resistance protein (LRP), and has shown to
interact with the estrogen receptor. The protein
is part of a very large vault ribonucleoprotein
complex present in all eurkaryotic cells and its
structure and protein composition is highly
conserved. Because of the size, shape, and
protein and RNA composition of this complex,
the particles are different from other
ribonucloproteins. This antibody can be applied
for the detection of MVP/LRP in a large number
of eukaryotic cells including the MCF-7 and Hela
tumor cell lines. It is especially applicable to
study the association of estrogen receptor with
vaults and the study of the mechanism of action
of estrogenic hormones.
Immunogen:
A BALB/c mouse was immunized with 5 μg of
affinity-purified nuclear extract proteins. Spleen
cells were fused with equal number of Sp0Ag-14
myeloma cells. Cells whose supernatant
showed a positive signal for a 104 kDa band
were selected and cloned by limited dilution.
Ig Isotype:
Mouse IgG1
Species Reactivity:
Human. Others not tested.
Format:
100 μg of purified monoclonal antibody.
Storage:
Store at 4°C short term, -20°C long term.
Aliquot to avoid freeze/thaw cycles.
Applications:

Immunohistochemistry: Frozen sections.

Western blot
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.