TRAIL小鼠ELISA試劑盒
產品名稱： TRAIL小鼠ELISA試劑盒
英文名稱： Apo 2 ligand APO2L CD253 Protein TRAIL TL2 TNF related apoptosis inducing ligand Tumor necrosis factor ligand superfamily member 10
產品編號： ab193730
產品價格： null
產品產地： 英國
品牌商標： abcam
更新時間： null
使用范圍：
- 聯系人 :
- 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
- 郵編 :
- 所在區域 : 廣東
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- 檢測方法Colorimetric
- 精確度
批次內 樣品 n Mean SD CV% overall ? ? ? < 10% ? - 樣品類型
Cell culture supernatant, Serum, Plasma
- 檢測類型Sandwich (quantitative)
- 靈敏度
2 pg/ml
- 范圍
2 pg/ml - 600 pg/ml
- 復檢
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特定樣本復檢結果 樣品類型 平均% 范圍 Serum = 97.38 91% - 104% Plasma = 88.25 76% - 104% Cell culture media = 122.5 115% - 129% ?
- 實驗步驟One step assay
- 種屬反應性
與反應:?Mouse
- 產品概述
Abcam’s TRAIL (TNF-related apoptosis-inducing ligand) Mouse ELISA Kit (ab193730) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TRAIL in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse TRAIL coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TRAIL present in the samples. The wells are washed and biotinylated anti-mouse TRAIL antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TRAIL bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 經測試應用Sandwich ELISAmore details
- 平臺Pre-coated microplate (12 x 8 well strips)
性能
- 存放說明Store at -20°C. Please refer to protocols.
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組件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200μl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated mouse TRAIL detection antibody 2 vials mouse TRAIL standards 2 vials Pre-coated mouse TRAIL Microplate 1 unit Stop Solution :sulfuric acid. 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - 研究領域
- 相關性TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
- 細胞定位Cell Membrane; single-pass type II membrane protein.
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別名
- Apo 2 ligand
- APO2L
- CD253
- Protein TRAIL
- TL2
- TNF related apoptosis inducing ligand
- Tumor necrosis factor ligand superfamily member 10
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