NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation for Illumina and Oxford Nanopore Technologies sequencing. The V3 ARTIC primers have been balanced, using methodology developed at NEB based on empirical data from sequencing. In combination with optimized reagents for RT-PCR, the kits deliver improved uniformity of amplicon yields from gRNA across a wide copy number range.  

If shorter inserts are preferred, the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (NEB #E7658) generates libraries with ~150 bp inserts, compatible with 2 x 75 bp sequencing.
Information on primer variant overlaps can be found here.
Streamlined, high-efficiency protocol.
Effective with a wide range of viral genome inputs (10-10,000 copies).
Improved uniformity of SARS-CoV-2 genome coverage depth with a more balanced primer pool.
No requirement for amplicon normalization prior to library preparation.
Library inserts in the 400 bp range.
Compatible with 2 x 250 Illumina sequencing.
Includes NEBNext Sample Purification Beads (SPRIselect).
Adaptors and primers available separately; For a 384 unique dual index format, please contact us.
For a bulk format of the kit (2,304 reactions) please contact us.