MAPK1 (K54R) (Human) Recombinant Protein
產品名稱: MAPK1 (K54R) (Human) Recombinant Protein
英文名稱: MAPK1 (K54R) (Human) Recombinant Protein
產品編號: P5679
產品價格: 0
產品產地: 臺灣
品牌商標: Abnova
更新時間: null
使用范圍: null
亞諾法生技股份有限公司(Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
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- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Human MAPK1 (NP_002736.3, 1 a.a. - 360 a.a.) K54R mutant full-length recombinant protein with GST tag expressed in Escherichia coli.
- Theoretical MW (kDa):
- 69
- Form:
- Liquid
- Preparation Method:
- Escherichia coli expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 96 % by SDS-PAGE/CBB staining
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Modified Erktide. ATP : 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE

- Note:
- Result of activity analysis
-

- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 5594
- Protein Accession#:
- NP_002736.3
- Gene Name:
- MAPK1
- Gene Alias:
- ERK,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk
- Gene Description:
- mitogen-activated protein kinase 1
- Omim ID:
- 176948
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000174492,extracellular signal-regulated kinase 2,extracellular signal-regulated kinase-2,mitogen-activated protein kinase 2,protein tyrosine kinase ERK2
- Gene Pathway
- Acute myeloid leukemia
- Adherens junction
- Alzheimer's disease
- Axon guidance
- B cell receptor signaling pathway
- Bladder cancer
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Colorectal cancer
- Dorso-ventral axis formation
- Endometrial cancer
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- Insulin signaling pathway
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Melanoma
- mTOR signaling pathway
- Natural killer cell mediated cytotoxicity
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prion diseases
- Prostate cancer
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- T cell receptor signaling pathway
- TGF-beta signaling pathway
- Thyroid cancer
- Toll-like receptor signaling pathway
- Type II diabetes mellitus
- Vascular smooth muscle contraction
- VEGF signaling pathway
