Anti-BRaf (V600D) Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-BRaf (V600D) Mouse Monoclonal Antibody

Anti-BRaf (V600D) Mouse Monoclonal Antibody

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產品名稱： Anti-BRaf (V600D) Mouse Monoclonal Antibody

英文名稱： Anti-B-Raf (V600D) Mouse Monoclonal Antibody

產品編號： 26184

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IF、IHC

武漢紐斯特生物技術有限公司
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B-Raf (V600D) [26184]
[100 μL]		 ?

Catalog Number:??26184
Gene Symbol:??BRAF, BRAF1, RAFB1
Description: ??Anti-B-Raf (V600D) Mouse Monoclonal Antibody
Background: ??B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen: ??A synthetic peptide from the internal region of B-Raf (V600D), human origin.
Applications: ??ELISA, WB, IF, IHC
Recommended Dilutions: ??
ELISA 1:1000-1:5000?
WB 1:500-1:2000?
IHC 1:100-1:200
Concentration: ???0.5 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??IgG
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes B-Raf V600D, but not wild-type B-Raf proteins from vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant B-raf proteins. Purified His-tagged B-Raf (V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-B-Raf (V600D) monoclonal antibody (Cat. # 26184).
Immunofluorescence: ??
Immunofluorescence of cells expressing B-Raf proteins with anti-B-Raf (V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-B-Raf (V600D) plasmid (right column), then fixed and stained with anti-B-Raf (V600D) monoclonal antibody (Cat. #26184).
Publications:
1.??Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
????Mod Pathol. 2013 Mar;26(3):414-20.
2.??Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
????Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.??Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
????human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.??Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
????human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.??BRAF V600E Mutation-Specific Antibody: A Review
????Seminars in Diagnostic Pathology