基本信息

產品名稱FITC標記鏈霉親和素英文名稱Streptavidin protein (FITC)規格或純度Streptavidin was conjugated with FITC under optimum conditions, and unconjugated Streptavidin and free FITC were removed.蛋白長度Full length proteinCarrier Free1% BSA偶聯標記FITC. Ex: 493nm, Em: 528nm來源Native

相關屬性

特點與好處Streptavidin (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.應用Flow Cytometry; IF;儲存緩沖液PBS (pH 7.4) buffer containing 1% BSA and 0.05% ProClin? 300 as a preservative.濃度Streptavidin Concentration: 1 mg/mL儲存溫度2-8°C儲存,避免反復凍融運輸條件冰袋運輸穩定性與儲存Shipped at 4℃. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

常規信息

英文別名FITC Streptavidin; Streptavidin-FITC; FITC-streptavidin conjugate; SAv-FITC; Streptavidin Fluorescein Conjugated; Streptavidin-Fluorescein Isothiocyanate;別名FITC Streptavidin; Streptavidin-FITC; FITC-streptavidin conjugate; SAv-FITC; Streptavidin Fluorescein Conjugated; Streptavidin-Fluorescein Isothiocyanate; 鏈霉親和素-FITC 來源于阿維丁鏈霉菌; 鏈霉親和素-熒光素異硫*酸酯 (FITC) 偶聯物; FITC鏈霉親和素;

一般描述

背景

鏈霉親和素（Streptavidin）是由streptomyces avidinii菌分泌的四聚體蛋白，可以高度特異性地和生物素（Biotin）結合，結合常數為10?1? mol/L，一分子 Streptavidin 可以結合 4 個 Biotin 分子，這種結合是目前自然界已知最強的非共價作用。 Streptavidin 和雞蛋清來源的親和素（Avidin）相比，兩者與 Biotin 結合的親和力高度相似性， 但Streptavidin在中性條件下幾乎不帶電荷，因此Streptavidin的非特異性結合比Avidin低很多， 檢測時的非特異性背景也就少很多。Streptavidin 可以用于 Biotin 標記的抗體、核酸、蛋白或其他生物素標記分子的檢測。Streptavidin (FITC)產品是用高純度的 Streptavidin 和高純度的 FITC 染料共價連接，并去除游離FITC。FITC標記的鏈霉親和素是間接法熒光染色中一種非常有用的二抗，可以高親和性地結合生物素化抗體。FITC由488nm的激光激發，可以在最大波長為520nm處檢測到其發射光。

推薦工作濃度

Flow cytometry: 1:500-1:5000

IF: 1:50-1:500

Background

Streptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10?1? mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Unlike egg-white avidin, which has a net positive charge at neutral pH and contains about 7% carbohydrate, streptavidin has almost no net charge at neutral pH, does not contain carbohydrate, and exhibits lower non-specific background. Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules. This FITC-streptavidin conjugate was prepared by highly purified Streptavidin and free FITC was removed. Streptavidin (FITC) is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Excitation at 488nm light leads to a fluorescence emission maximum of 520 nm.

Recommended Usage：

Every lot of Streptavidin-FITC is tested by flow cytometry using biotinylated primary antibodies. From this testing it is recommended that between 0.02 and 0.25 μg of streptavidin be used per 106 cells in a 100 μl staining volume.

圖片

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .

Flow-Streptavidin protein (FITC) (rp155876)

Flow-Streptavidin protein (FITC) (rp155876)

BALB/c splenocytes stained with Biotin-CD45R antibody, followed by Streptavidin protein (FITC) (rp155876) at 1/2500 dilution.