• 特異性ab183075 detects endogenous level of total 14-3-3 theta protein.
• 經測試應用WB,?IHC-Pmore details
• 種屬反應性
  與反應:?Mouse, Human
  預測可用于:?Rat, Cow, Orangutan
• 免疫原

  Synthetic peptide within Human 14-3-3 theta aa 140-154. The exact sequence is proprietary. Genbank: NP_006817
  Sequence:

  QTIDNSQGAYQEAFD

  
  Database link:?P27348

  ?

  Run BLAST with?Run BLAST with?
• 陽性對照
  • Mouse brain, Human lymphoma and Human colon cancer tissue lysates; Human A549 and mouse NIH 3T3 cell lysates; Human tonsil tissue

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存???溶液pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: 50% Glycerol, 49% PBS
  
  PBS is without Mg²⁺?and Ca²⁺
• 濃度100 μl 濃度為 0.6 mg/ml?
  
• 純度Immunogen affinity purified
• 克隆多克隆
• 同種型IgG
• 研究領域
  • Signal Transduction
  ?
  • Adapters
  ?
  • Cytoplasmic

  • Stem Cells
  ?
  • Signaling Pathways
  ?
  • Wnt
  ?
  • 14-3-3

應用

靶標

• 細胞定位Cytoplasmic
• 數據庫鏈接
  • Entrez Gene: 768311?Cow
  • Entrez Gene: 10971?Human
  • Entrez Gene: 22630?Mouse
  • Entrez Gene: 100171925?Orangutan
  • Entrez Gene: 25577?Rat
  • Omim: 609009?Human
  • SwissProt: Q3SZI4?Cow
  • SwissProt: P27348?Human

  see all
• 別名
  • 14-3-3 antibody
  • 14-3-3 protein T-cell antibody
  • 14-3-3 protein tau antibody

  see all

Anti-14-3-3 theta antibody 圖像

• 

  Western blot - Anti-14-3-3 theta antibody (ab183075)
  All lanes :?Anti-14-3-3 theta antibody (ab183075) at 1/300 dilution
  
  Lane 1 :?mouse brain tissue lysate
  Lane 2 :?Human A549 cell lysate
  Lane 3 :?Human lymphoma tissue lysate
  Lane 4 :?mouse NIH 3T3 cell lysate
  Lane 5 :?Human colon cancer tissue lysate
  
  Lysates/proteins at 40 μg per lane.
  
  Secondary
  Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  developed using the ECL technique
  
  Predicted band size :?28 kDa
  
  
  Exposure time :?5 seconds
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (ab183075)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta?with ab183075?at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)