ZYCY10P3S2T E.coli
ZYCY10P3S2T E.coli Minicircle producer competent cells have
been prepared and tested by a modification of the procedure of
Kay (Kay MA, He CY, Chen ZY. 2010, Nature Biotechnology).
These cells are suitable for the cloning of minicircle-based
plasmids. ZYCY10P3S2T E.coli minicircle producer cells are
derived from a BW27783 bacterial strain that stably expresses a
set of inducible minicricle-assembly enzymes, ФC31 integrase and
I-SceI homing endonuclease. This bacterial strain produces
purified minicircles in a time frame and quantity similar to those of
routine plasmid DNA preparation, making it feasible to use
minincircles in place of plasmids in mammalian transgene
expression studies.
Note: Other competent E. coli strains cannot be substituted
for minicircle production as they do not have the appropriate
genome modifications to produce minicircles.