PEI是歷史上繼多聚賴氨酸之后發現的第二種聚合物轉染試劑。PEI能將DNA縮合成帶正電荷的微粒，這些微?？梢责ず系綆в胸撾姾傻募毎砻鏆埢⑼ㄟ^胞吞作用進入細胞。一旦進入細胞，胺的質子化導致反離子大量涌入以及滲透勢降低。上述變化導致的滲透膨脹使囊泡釋放聚合物與DNA形成的復合物（polyplex）進入細胞質。復合物拆解后，DNA就能自由的融合到細胞核中。聚Ethylene亞胺（Polyethylenimine，PEI）是一種具有較高的陽離子電荷密度的有機大分子，每相隔二個碳個原子，即每第三個原子都是質子化的氨基氮原子，使得聚合物網絡在任何pH下都能充當有效的質子海綿(proton sponge)體。

轉染試劑線性PEI MAX溶液的配制

線性聚乙烯亞胺鹽酸鹽(Linear Polyethylenimine Hydrochloride)是線性化聚乙烯亞胺PEI?25,000的升級產品，是一種功能更為強大的高效瞬時轉染試劑。與PEI 25000的區別在于：1）完全水解（去乙?；?）PEI 25000的鹽酸鹽形式，具更高的水溶特性 3）含更長連續性乙烯亞胺片段，其質子化氮水平比PEI25000提高11%以上。

另PEI MAX 40K比PEI 25K更易于使用，并且更高效。PEI 25K轉染溶液通常需要幾個小時才能制備，而PEI MAX 40K可以在兩個小時內轉化為即用型溶液。而且PEI 25K含有4-11%的殘余丙?；煞乐咕酆衔镏麈溑cDNA強烈結合。PEI MAX 40K完全去乙?；慕Y構意味著每批產品的表現始終如一。

下面將主要介紹polysciences 線性PEI MAX(#24765)的產品特性及其溶液的配制過程。

1.產品特性:

產品名稱：轉染級線性聚乙烯亞胺鹽酸鹽(PEI?MAX)(Linear Polyethylenimine Hydrochloride)

2.溶液配制

此指導說明書是經polysciences公司研究，測試及其客戶反饋的后編寫的。下面推薦一種簡單適用而高效的方法，講述如何配制濃度為1mg/mL的轉染試劑的配制。

材料：

1g #24765、1L 純水（Milli-Q?純水，注射用水（WFI），或類似的生物級水）、1N OH化鈉、25mL無菌塑料吸管、一次性0.1μm,?0.2μm, 0.22μm PES真空無菌過濾器、無菌高聚乙烯或聚丙烯試劑瓶。

器材：

1 L 玻璃燒杯、1 L玻璃量筒、pH計、移液器、磁力攪拌棒、帶油管的真空泵

配制過程：

1g #24765——900mL水溶解——攪拌、加熱（60-80°C）——加1N NaOH調pH到6.9-7.1——移液到量筒，加水補足至1L——真空過濾消毒——分裝，4°C保存。

備注：加熱或加酸都有助于PEI溶解。#24765比#23966易溶，可適當少量加酸，詳細用量可試加一點攪拌看看，足夠溶解就行，不一定需要加到pH那么低。

聚乙烯亞胺(PEIs)polysciences代理商現貨

PEI MAX - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000)

View pricing for:?Americas/Asia,?Europe,?Taiwan

DESCRIPTIONAdd to CartBulk Quote

PEI MAX 40K (also known as PEI 22K in free base) is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Each year, more researchers and companies turn to Polysciences PEI to gain an edge in their critical work. Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.

PEI MAX 40K is easier to use and offers consistently higher titers than PEI 25K. PEI 25K transfection solutions typically take several hours to prepare, while PEI MAX 40K can be converted to a ready-to-use solution in under two hours. Additionally, PEI 25K contains 4-11% residual propionyl groups, which prevents the polymer backbone from strongly binding to DNA. PEI MAX 40K’s fully depropionylated structure means each batch performs consistently higher.

Comparison of PEI 25K (#23966) and PEI MAX 40K (#24765).?
Method: 10e6/mL HEK293 cells in 50 mL FS transfected with IgG64 plasmid pair. PEI:DNA 4:1. Samples taken 120 hpt. Quantified with Thermo Fisher #23310. N=4 each. Error bar = standard deviation.

A pre-made sterile-filtered solution of PEI MAX 40K is available as our?Transporter 5? Transfection Reagent.

CAS#:?

49553-93-7

Molecular Weight:?

40,000 (~22,000 free base)

Soluble In:?

Cold and room temperature water?
Insoluble in:?Common organic solvents (ethanol, acetone, tetrahydrofuran)

Appearance:?

White to off-white free flowing solid

Reference(s):

Baranyi, L. et al.?Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors.?Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Delafosse, L., Xu, P. & Durocher, Y.?Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells. Journal of Biotechnology 227, 103–111 (2016). doi:10.1016/j.jbiotec.2016.04.028

** Gutiérrez-Granados, S., Cervera, L., Segura, M. de las M., W?lfel, J. & Gòdia, F. Optimized production of HIV-1 virus-like particles by transient transfection in CAP-T cells. Applied Microbiology and Biotechnology 100, 3935–3947 (2016). doi:10.1007/s00253-015-7213-x

Kobayashi, S., Yoshii, K., Hirano, M., Muto, M. & Kariwa, H.?A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells.?Journal of Virological Methods 240, 14–20 (2017). doi:10.1016/j.jviromet.2016.11.006

** Longo, P. a, Kavran, J. M., Kim, M. & Leahy, D. J.?Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI).?Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.

Mann JF, McKay PF, Arokiasamy S, Patel RK, Klein K, Shattock RJ. (2013).?Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.

** Stuible, M. et al.?Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells. Journal of Biotechnology 281, 39–47 (2018). doi:10.1016/j.jbiotec.2018.06.307

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005).?Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung.?Proc Natl Acad Sci U S A.?102(16):5679-84.

** Contains particularly useful information.

TSCA?

Hazards:?

Irritant

Handling:?

Glove, chemical goggles & fume hood

Storage:?

Store at room temperature

MSDS / TECHNICAL DATA SHEETS / PRODUCT LITERATURE

• MSDSMaterial Safety Datasheet 24765 (PDF)
• DATAFT-IR Spectrum
• DATAExample COA
• LITBSE/TSE Statement
• LITTransfection Reagent Preparation and Storage Recommendations

RELATED PRODUCTS

1.Transporter 5? Transfection Reagent

2.Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K)