Form of Antibody: Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Store at -20℃/1 year?
Clonality: Monoclonal?
Immunogen: The antiserum was produced against synthetic peptide HHHHHH coupled to KLH.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity/Sensitivity: His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.
Reactivity: All
Applications: WB 1:5000 IP 1:200 IF 1:1000?
The optimal working dilution should be determined by the end user.
References:

WB:All lanes : His-Tag Mouse Monoclonal antibody A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with his-tag protein C: IP (PBS + anti-HIS mAb 4℃ overnight) D: IP (HEK293 transfected+protein G+anti-HIS mAb 4℃ overnight)) E: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) F: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

IF:

Immunofluorescence analysis of 293T cells transfected with a His-tagged protein, using His-Tag Mouse Monoclonal antibody at a 1:2000 dilution (blue DAPI, red anti-His).