熱休克轉錄因子2抗體
產品名稱： 熱休克轉錄因子2抗體
英文名稱： HNF1A
產品編號： 1405
產品價格： null
產品產地： 上海
品牌商標： 雅吉
更新時間： null
使用范圍： WB ELISA IHC-P IHC-F IF
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| 中文名稱 | 熱休克轉錄因子2抗體 |
| 別????名 | Heat shock factor protein 2; Heat shock transcription factor 2; HSF 2; HSF2; HSTF 2; HSF2_HUMAN.?? |
| 研究領域 | 腫瘤??細胞生物??免疫學??神經生物學??細胞凋亡??轉錄調節因子?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Rat,? (predicted: Mouse,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 59kDa |
| 細胞定位 | 細胞核?細胞漿? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human HSTF2:401-536/536? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]. Function: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Subunit: DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer. Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation. Similarity: Belongs to the HSF family. SWISS: Q03933 Gene ID: 3298 Database links: Entrez Gene: 3298?Human Entrez Gene: 15500?Mouse Omim: 140581?Human SwissProt: Q03933?Human SwissProt: P38533?Mouse Unigene: 158195?Human Unigene: 261395?Mouse Unigene: 447362?Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 轉錄調節因子（Transcriptin Regulators） 熱休克轉錄因子在熱休克反應中的主要功能,是在熱休克基因的表達過程中啟動基因轉錄過程,促進熱休克蛋白的表達. |
| 產品圖片 |  Sample:HepG2 Cell(Human)Lysate at 30 ug Primary: Anti- HSTF2 (bs-1409R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59kD  Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. |
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