Product: Anti-APRIL mAb, clone Sacha-1
Cat. No.: MC-091 (100 μg)
Synonyms:
A-Proliferation-inducing Ligand, CD256, TNFSF
13.
Specificity:
Recognizes human and mouse APRIL.
Species Reactivity:
Human and mouse. Others species not tested.
Ig Isotype:
Rat IgG2a
Immunogen:
Recombinant human APRIL (aa 105-250) fused
to human ACRP30headless (aa 16-108).
Format:
100 μL of a 1 mg/mL solution of monoclonal
antibody purified from concentrated hybridoma
tissue culture supernatant. Antibody is in PBS
with 0.02% sodium azide. >95% purity as
determined by SDS-PAGE.
Storage:
Store at 4°C. Do not freeze/thaw.
Applications:

Immunocytochemistry

Flow cytometry: Excellent for mouse APRIL,
also stains human APRIL.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Figure 1: Immunostaining of HEK-293T cells transfected with
a mouse APRIL expression plasmid (left panels) or mock
transfected (right panel).
Method: 3 days after transfection of cells with the indicated
constructs, cells were fixed with acetone. Slides were
blocked with IgG, and incubated for 1 hour with 5 μg/mL anti-
APRIL (MC-091) or control mouse IgG (isotype control) in
1% BSA/1x PBS for 1 hour. After washes with PBS, samples
were incubated with the secondary Ab for 1 hour, washed in
PBS and detected with Streptavidin-Biotin/HRP and AEC.
Figure 2: Monoclonal antibody to APRIL, MC-091, detects
membrane-bound human APRIL by FACS.
Method: HEK 293T cells (5 x 105) were mock transfected
(thin line) or transfected with an expression plasmid enabling
surface expression of mouse APRIL (thick line). Cells were
incubated on ice for 30 minutes in 50 μL FACS buffer (PBS,
5% fetal calf serum, 0.02% azide) containing 1 μg/mL of anti-
APRIL (MC-091). After washing in FACS buffer, PEconjugated
antibody to rat IgG was added. Cells were
incubated on ice for 30 minutes, washed and then analyzed
by flow cytometry.