Product: Anti-Bnip3L (IN) pAb
Cat. No.: PC-148 (100 μg)
Description:
Members in the Bcl-2 family are critical
regulators of apoptosis by either inhibiting or
promoting cell death. Bcl-2 homology 3 (BH3)
domain is a potent death domain. BH3 domain
containing pro-apoptotic proteins, including Bad,
Bax, Bid, Bik, Hrk, Nip3, and Bim, form a
growing subclass of the Bcl-2 family. A novel
BH3 domain containing protein was recently
identified and designated Bnip3L, Bnip3a, and
Nix (for Nip3-like Protein X). Bnip3L/Bnip3a/Nix
is a homolog of the E1B 19K/Bcl-2 binding and
pro-apoptotic protein Bnip3. Overexpression of
Bnip3L induces apoptosis. Bnip3L interacts with
and overcomes suppresses by Bcl-2 and Bcl-xL.
Bnip3L is localized in mitochondria. The
messenger RNA of Bnip3L is ubiquitously
expressed in human tissues. Bnip3L and Bnip3
form a new subfamily of the pro-apoptotic
mitochondrial proteins.
Specificity:
Recognizes Bnip3L. Detects a band of ~40 kDa
by Western blot.
Species Reactivity:
Human and mouse. Others not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
Synthetic peptide corresponding to aa 77-92
(DAQHESGQSSSRGSSH) of human origin,
which are identical to those of mouse Bnip3L.
Format:
100 μg of immunoaffinity chromatography
purified rabbit IgG at 1 mg/mL in PBS containing
0.02% sodium azide.
Positive Control:
K562 cell lysate.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 – 1 μg/mL.

Immunohistochemistry: Use at 2 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of Bnip3L in K562 whole cell
lysate in the absence (A) or presence (B) of immunogenic
peptide with anti-Bnip3L (IN) at 1 μg/mL. The approximate
40 kDa top band represents monomer of Bnip3L.
Figure 2: Immunohistochemical staining of human kidney
tissue using Bnip3L antibody at 2 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any damage,
personal injury, or economic loss caused by this
produc