Size??100ug
Cat code??200608
Species Cross-reactivity??H,M,Mk,G,Hm,R
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??55kDa
Sensitivity??This antibody detects endogenous levels of Tubulin beta and does not cross-react with related proteins.
Immunogen??Purified recombinant human Tubulin beta protein fragments expressed in E.coli.
UniProt Number??P07437
GeneBank ID??NM_178014
Subcellular location??Cytoplasm > cytoskeleton.
Formulation??Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername??Alpha tubulin;Alpha-tubulin ubiquitous;K ALPHA 1;TBA1B_HUMAN;TUBA1B;TUBB;TUBB1;TUBB2;TUBB2A;TUBB5;Tubulin alpha-1B chain;Tubulin alpha-ubiquitous chain;Tubulin beta 1b;Tubulin beta 2A;Tubulin beta;Tubulin K-alpha-1.
Gene_symbol??M40; TUBB1; TUBB5; OK/SW-cl.56
Summary??null
UniPort summary  Function??Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Application Images :??
Western blot detection of TUBB in COS7,LNCap,MCF7&C2C12 cell lysates and using TUBB antibody (1:5000 diluted). Predicted band size: 55KDa. Observed band size: 55KDa.
Immunocytochemistry staining of Hela cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-TUBB antibody (dilution 1:1000).
TUBB antibody’s stability test under 37℃ 50 hours compare to 4℃ 50 hours
Western blot detection of TUBB antibody in goat intestine,goat skeletal muscle,goat spleen & goat lung cell lysates using TUBB antibody (1:5000-1:10000 diluted).
Predicted band size: 55KDa.
Observed band size: 55KDa.
Western blot detection of TUBB antibody in Goat skeletal muscle,CHO-k1,COS7,C6,3T3 and K562 cell lysates using TUBB antibody (1:5000 diluted).Predicted band size:55KDa.Observed band size:55KDa.Exposure time:10sec.